Current-clamp experiments on an identified neurone have demonstrated the presence of L-glutamate receptors in the insect central nervous system. The cell body of the fast coxal depressor motor neurone (Df) in the metathoracic ganglion of the cockroach Periplaneta americana exhibits a hyperpolarizing response to L-glutamate, accompanied by an increase in membrane conductance. The response is dep...

The protective effect of a L-type calcium channel blocker, nimodipine, on cell injury induced by oxygen-glucose deprivation (OGD) in PC12 cells was investigated. PC12 cells were exposed to in-vitro oxygen-glucose deprivation (30 minutes and 60 minutes respectively) in the presence or absence of nimodipine (10mM/L) in three different time schedules (pre-24h, pre-3h and concurrently). Cellular vi...

In the presence of pipecolate, Pseudomonas putida P2 (ATCC 25571) converts cu-amino[6-14C]adipate to radioactive glutamate with a 14C distribution of 11% and 80 % in carbons 1 and 5, respectively. This labeling pattern, when considered with previous data, indicates that a-aminoadipate is an obligate intermediate in the metabolism of pipecolate to glutamate. Some of the 14C in carbon 1 results f...

BACKGROUND Depression of glutamate-mediated excitatory transmission and potentiation of gamma-aminobutyric acid (GABA)-mediated inhibitory transmission appear to be primary mechanisms by which general anesthetics produce anesthesia. Since effects on transmitter transport have been implicated in anesthetic actions, the authors examined the sensitivity of presynaptic glutamate and GABA transporte...

In vivo studies with L-[13N]glutamate in the Walker 256 carcinosarcoma implanted under the renal capsule of female Sprague-Dawley rats demonstrate that uptake of glutamate and the rate of incorporation of the nitrogen label from this amino acid into metabolites is slower in the tumor than in nontumorous kidney tissue. Glutamate dehydrogenase, glutaminase, and alanine aminotransferase activities...

Using whole cell patch-clamp technique and immunocytochemistry on adult dorsal unpaired median (DUM) neurons isolated from the cockroach Periplaneta americana CNS, we reported the characterization of a native mGluR, sharing pharmacological properties with vertebrate metabotropic glutamate receptor III (mGluRIII) that regulated voltage-dependent sodium current (I(Na)). The global I(Na) was disso...

Ethnopharmacological relevance: Ocimum basilicum L. (Lamiaceae) is a medicinal plant known in Cameroon for the treatment of many ailments like anxiolytic, antispasmodic and mental disorders. Aim study: The aim this study to evaluate neuroprotective effects basilicum. Materials methods: T-maze test open field assay oxidative stress parameters were used detecting its capacity protect neurons agai...