background: visceral leishmaniasis is caused by leishmania infantum , transmitted to humans by bites of phle­botomine sand flies and is one of the most important public health problems in iran. to identify the vector(s), an investiga­tion was carried out in bilesavar district, one of the important foci of the disease in ardebil province in northwest­ern iran, during july - september 2008 . meth...

background: since pars abad district had been known as a focus of visceral leishmaniasis (vl) in ardabil province but the prevalence of the disease in nomadic tribes has not been determined, thus, this study was conducted. methods: this descriptive cross- sectional study was conducted on children up to 12yr old of nomadic tribes from pars abad county, ardabil province, iran in 2015. for each in...

A gp63PCR method was evaluated for the detection and characterization of Leishmania (Leishmania) (L.) parasites in canine lymph node aspirates. This tool was tested and compared to other PCRs based on the amplification of 18S ribosomal genes, a L. infantum specific repetitive sequence and kinetoplastic DNA minicircles, and to classical parasitological (smear examination and/or culture) or serol...

BACKGROUND Leishmania infantum recently has been identified as a possible agent of cutaneous leishmaniasis (CL). This species has been isolated from cutaneous lesions of patients from the Mediterranean Basin. However, little is known about the clinical, biological, or therapeutic features of this newly recognized CL. OBSERVATIONS Six patients aged 9 months to 85 years in southeastern France w...

Visceral leishmaniasis, caused by Leishmania (L.) donovani and L. infantum protozoan parasites, can provoke overwhelming and protracted epidemics, with high case-fatality rates. An effective vaccine against the disease must rely on the generation of a strong and long-lasting T cell immunity, mediated by CD4+ TH1 and CD8+ T cells. Multi-epitope peptide-based vaccine development is manifesting as...

Dear Editor, Visceral leishmaniasis (VL) is the most severe form of a neglected tropical diseases group transmitted by phlebotomine sandflies harboring promastigotes forms of donovani complex Leishmania species with distinct eco-epidemiological patterns. The anthroponotic L. donovani in East Africa and India; and the zoonotic L. infantum in Europe and North Africa and in America after its intro...

Background: Lipophosphoglycan 3 (LPG3) is required for the LPG assembly, a well known virulent molecule. In this study, the LPG3 gene of the lizard and mammalian Leishmania species were cloned and sequenced. A three-dimensional structure (3D) for the target sequence was also predicted by comparative (homology) modeling. Materials and Methods: An optimization PCR amplification was performed o...

Leishmaniasis represents a serious threat to the health as one of the most important neglected tropical diseases as designated by the World Health Organization. The disease is endemic in 82 countries, among them Tunisia is an indigenous area for cutaneous Leishmaniasis. In a previous work, two tritepenic acids namely oleanolic and maslinic acids have been isolated from olive leaf extract. In th...

Despite the broad distribution of leishmaniasis among Iranians and animals across the country, little is known about the genetic characteristics of the causative agents. Applying both HSP70 PCR-RFLP and sequence analyses, this study aimed to evaluate the genetic diversity and phylogenetic relationships among Leishmania spp. isolated from Iranian endemic foci and available reference strains. A t...