Flavocytochrome b2 from Saccharomyces cerevisiae acts physiologically as an L-lactate dehydrogenase. Although L-lactate is its primary substrate, the enzyme is also able to utilize a variety of other (S)-2-hydroxy acids. Structural studies and sequence comparisons with several related flavoenzymes have identified the key active-site residues required for catalysis. However, the residues Ala-198...

Coronavirus is a new pandemic disease that began in Wuhan, China, and has since spread over the globe. The number of COVID-19 cases reported daily Iraq slowly increased. This study aims to investigate effect Covid-19 on normal range White Blood Cells (WBC), D-Dimer, Lactate Dehydrogenase (LDH). looked at 65 patients who had tested positive for SARS-CoV-2 using polymerase chain reaction analysis...

abstract background: athletes’ recovery is important in improving their performance. nutritional strategies can be effective in enhancing recovery rate. choosing the best food items in appropriate intervals can play effective roles in resynthesis of fuels and recovery of muscle injury. beverage micro and macronutrient content are helpful in fuel restoration. in this study, we assess the effects...

This work describes a novel L-lactate biosensor based on the immobilization of dehydrogenase enzyme screen-printed electrode modified with ternary composite gold nanoparticles, electrochemically-reduced graphene oxide, and poly (allylamine hydrochloride). The was stabilized by crosslinking glutaraldehyde. Applied working potential, pH NAD+ concentration were optimized. reports specific sensitiv...

background: parasite lactate dehydrogenase ( p ldh) is extensively employed as malaria rapid diagnostic tests (rdts). moreover, it is a well-known drug target candidate. however, the genetic diversity of this gene might influence perfor­mance of rdt kits and its drug target candidacy. this study aimed to determine polymorphism of p ldh gene from iranian isolates of p. vivax and p. falciparum. m...

Two His residues, His-205 and His-296, in Lactobacillus plantarum D-lactate dehydrogenase are highly conserved in the D-isomer-specific 2-hydroxyacid dehydrogenase family, suggesting that they are candidates for the catalytic His in the enzyme. The substitution of His-296 with Tyr by means of site-directed mutagenesis induced a drastic decrease in the reaction rate, while a His-205 to Tyr subst...

Lactococcus lactis can undergo respiration when hemin is added to an aerobic culture. The most distinctive feature of lactococcal respiration is that lactate could be consumed in the stationary phase concomitantly with the rapid accumulation of diacetyl and acetoin. However, the enzyme responsible for lactate utilization in this process has not yet been identified. As genes for fermentative NAD...

In the light of the occurrence of L-lactate dehydrogenase inside the mitochondrial matrix, we looked at whether isolated rat liver mitochondria can take up and metabolize L-lactate, and provide oxaloacetate outside mitochondria, thus contributing to a partial reconstruction of gluconeogenesis in vitro. We found that: (1) L-lactate (10 mM), added to mitochondria in the presence of a cocktail of ...

BACKGROUND Although the blood lactate-to-pyruvate (L:P) molar ratio is used to distinguish between pyruvate dehydrogenase deficiency (PDH-D) and other causes of congenital lactic acidosis (CLA), its diagnostic accuracy for differentiating between these 2 types of CLA has not been evaluated formally. METHODS We conducted a retrospective study of all patients followed for mitochondrial diseases...