Objective Cutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods. Methods Novy-MacNeal-Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal...

This work describes an analytical platform based on semi-high-resolution antileishmanial profiling combined with hyphenation of high-performance liquid chromatography - high-resolution mass spectrometry - solid-phase extraction - nuclear magnetic resonance spectroscopy, i.e., semiHR-antileishmanial assay/HPLC-HRMS-SPE-NMR. The platform enables fast pinpointing of HPLC peaks representing Leishma...

Continuando su estudio del "fen6meno isopatico" en la piel de pacientes con lepra lepromatosa cuando se Ie inyecta varias substancias, los autores informan los resultad03 de experimentos con BCG y Lei.~hmania tropica. Con BCG en 21 pacientes, 8 de ellos sin lesiones activas y todos recibiendo quimioterapia, las lesiones producides demostraron conglomerados de histiocitos 0 lesiones prelepromato...

Mucosal leishmaniasis is a major problem in Latin America but has been rarely noticed in our region. Although there have been a few reports of mucosal involvement especially in the oral cavity from Southwest Iran, yet none have been presented from the Northeast where Leishmania tropica is the major concern. We report a patient with endonasal leishmaniasis due to L. tropica, an extremely rare en...

In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. Cell proteins were analysed with 2-dimensional electrophoresis and differentially expressed proteins...

The induction of heat shock proteins in three species of Leishmania, L. tropica, L. enrietti, and L. donovani is reported. When cultures of promastigotes are shifted from 26 degrees C to 37 degrees C or 40 degrees C, the synthesis of proteins with apparent molecular weights of 88,000, 74,000, and 54,000 is stimulated. Actinomycin D added just prior to the shift prevented the appearance of these...

A sequence database was created for the Leishmania N-acetylglucosamine-1-phosphate transferase (nagt) gene from 193 independent isolates. PCR products of this single-copy gene were analyzed for restriction fragment length polymorphism based on seven nagt sequences initially available. We subsequently sequenced 77 samples and found 19 new variants (genotypes). Alignment of all 26 nagt sequences ...

background: characterization of leishmania parasites is necessary for epidemiological objectives such as documenting the distri­bution of predominant species and designing appropriate control measures. in this study, we aimed to identify leishma­nia species isolated from cutaneous leishmaniasis (cl) patients, using rapd-pcr method. methods: this descriptive, cross-sectional study was designed a...

General Considerations The most common etiologic agents of VL are 3 species of the L. donovani complex: Leishmania donovani donovani and Leishmania donovani infantum in the Indian subcontinent, Africa, China, the Mediterranean littoral, and the Middle East; and Leishmania donovani chagasi in South America. (Hereafter, organisms will be identified only by genus and species; eg, L. donovani for L...

Cutaneous leishmaniasis is one of the most important parasitic diseases, which are endemic in different parts of Iran. Leishmania major and L. tropica are the primary causative agents of this disease. The aim of the present study was to detect the multiple forms of L. major in lung. Ppromastigotes of L. major at stationary phase were injected to BALB/c mice. After 60 days, the different forms o...