Microbial degradation of cellulose and lignocelluloses derivatives has received considerable attention towards bioremediation in recent years. The biological significance of lignin combined with the commercial utility of lignocellulose has generated widespread interest in understanding the biochemistry of lignocellulose degradation which is carried out by one of the copper containing oxidative ...

Laccase production by solid state fermentation (SSF) using an indigenously isolated litter dwelling fungus Fusarium incarnatum LD-3 was optimized. Fourteen medium components were screened by the initial screening method of Plackett-Burman. Each of the components was screened on the basis of 'p' (probability value) which was above 95% confidence level. Ortho-dianisidine, thiamine HCl and CuSO(4)...

The reactions of nitric oxide (NO) with the oxidized and reduced forms of fungal and tree laccase, as well as with tree laccase depleted in type 2 copper, are reported. The products of the reactions were determined by NMR and mass spectroscopy, whereas the oxidation states of the enzymes were monitored by EPR and optical spectroscopy. All three copper sites in fungal laccase are reduced by NO. ...

Extracellular laccase was produced by Trametes versicolor IBL-04 using corn cobs as a substrate under pre-optimized culture conditions. A 64kDa laccase enzyme was purified and immobilized on calcium alginate beads using glutaraldehyde as a cross-linking reagent. Maximum enzyme immobilization efficiency (89%) was observed with 2-mm calcium-alginate beads that were developed using 4% (w/v) sodium...

Suitable substrates for laccase production by the thermotolerant basidiomycete Coriolus versicolor strain RC3 were screened using solid and liquid media. In liquid basal medium, 1%(w/v) rice bran as a carbon source was found to be the most efficient substrate for laccase production compared to 1%(w/v) glucose, wheat bran and rice straw meal. After 15 days cultivation at 37°C in shake flask cult...

To identify white rot fungi with high potential in biological pretreatment of lignocellulosic biomass, preliminary screening was carried out on plates by testing different strains for their ability to oxidize guaiacol and decolorize the dyes azure B and Poly R-478. Of the 86 strains screened, 16 were selected for secondary screening for their ligninolytic ability; however, low manganese peroxid...

Reduction of dioxygen catalyzed by laccase was studied at platinum nanoparticles (PtNPs) modified electrodes. PtNPs with laccase are directly fabricated on glassy carbon electrode (GCE) and indium tin oxide electrode (ITO) by simple process. The PtNPs/laccase modified ITO electrode surface has been studied in detail using atomic force microscopy (AFM). This PtNPs/laccase modified GCE effectivel...

Convenient expression systems for efficient heterologous production of different laccases are needed for their characterization and application. The laccase cDNAs lcc1 and lcc2 from Trametes versicolor were expressed in Pichia pastoris and Aspergillus niger under control of their respective glyceraldehyde3-phosphate dehydrogenase promoters and with the native secretion signal directing catalyti...

Degradation of residual lignin in kraft pulp by chemical bleaching is implicated in causing environmental pollution. The use of thermo- and alkali-tolerant bacterial laccases is considered to be important biological alternative to chemical processing. Laccases from Bacillus species have shown promise in this respect but their intracellular/spore bound presence make their industrial application ...

Ganoderma lucidum has a well-developed ligninolytic enzyme system, where laccase is the dominant and sometimes only synthesizing enzyme, and therefore could find an application in the delignification of abundant plant raw materials and in food, feed, paper, and biofuel production. The questions that provided the goals for the present study were whether the profile of G. lucidum laccase depends ...