Shyzophyllum commune IBL-06 produced 367 IU/mL of laccase in solid state bioprocess of banana stalk under optimum physical and nutritional parameters. The optimum SSF conditions were рH 4.5; temperature, 35°C; inoculum size, 3mL (10-10 mL); moisture content, 60%; C:N ratio in the medium, 15:1 (glucose and ammonium nitrate as additional carbon and nitrogen sources, respectively), ABTS (1mM), 1 m...

BACKGROUND In recent years, enzymatic-assisted removal of hazardous dyes has been considered as an alternative and eco-friendly method compared to those of physicochemical techniques. The present study was designed in order to obtain the optimal condition for laccase-mediated (purified from the ascomycete Paraconiothyrium variabile) decolorization of Acid Blue 92; a monoazo dye, using response ...

Free laccase has limitations for its use in industrial applications that require immobilization on proper support, to improve catalytic activity. Herein, the nanoparticles of magnetic iron oxide (Fe3O4) and copper ferrite (CuFe2O4) were successfully used as support free laccase, using glutaraldehyde a cross-linker. The conditions surface optimized reach maximum activity immobilized enzyme. synt...

Laccases are blue copper oxidases (E.C. 1.10.3.2) that catalyze the one-electron oxidation of phenolics, aromatic amines, and other electron-rich substrates with the concomitant reduction of O2 to H2O. A novel laccase gene pclac2 and its corresponding full-length cDNA were cloned and characterized from Phytophthora capsici for the first time. The 1683 bp full-length cDNA of pclac2 encoded a mat...

The white rot fungus Pycnoporus cinnabarinus was characterized with respect to its set of extracellular phenoloxidases. Laccase was produced as the predominant extracellular phenoloxidase in conjunction with low amounts of an unusual peroxidase. Neither lignin peroxidase nor manganese peroxidase was detected. Laccase was produced constitutively during primary metabolism. Addition of the most ef...

1-Hydroxybenzotriazole, violuric acid, and N-hydroxyacetanilide are three N-OH compounds capable of mediating a range of laccase-catalyzed biotransformations, such as paper pulp delignification and degradation of polycyclic hydrocarbons. The mechanism of their enzymatic oxidation was studied with seven fungal laccases. The oxidation had a bell-shaped pH-activity profile with an optimal pH rangi...

Introduction Laccase (benzenediol: oxygen: oxidoreductases, (EC. 1.10.3.2), phylogenitically old enzyme of oxidoreductase family and major secretome of many of the white rot fungi. They are capable of oxidizing diphenols, polyphenols, different substituted phenols, diamines, aromatic amines, benzenethiols, polyphenols and even some inorganic compounds (Bakkiyaraj et al., 2013). It has been wide...

Industrial effluents of textile, paper, and leather industries contain various toxic dyes as one of the waste material. It imparts major impact on human health as well as environment. The white rot fungus Pycnoporus cinnabarinus Laccase is generally used to degrade these toxic dyes. In order to decipher the mechanism of process by which Laccase degrade dyes, it is essential to know its 3D struc...

The aim of the present study reported here is to examine the production, purification and characterization of the enzyme laccase from the mushroom Hypsizygus ulmarius. The culture conditions were optimized for the maximum production of enzymes with different parameters such as incubation time, carbon, nitrogen sources. The enzyme was purified by ammonium sulphate precipitation and partially cha...

Indigo carmine is a typical recalcitrant dye which is widely used in textile dyeing processes. Laccases are versatile oxidases showing strong ability to eliminate hazardous dyes from wastewater. However, most laccases require the participation of mediators for efficient decolorization of indigo carmine. Here we describe the improvement of the decolorization ability of a bacterial laccase throug...