Antibiotic drug resistance among hospital and community acquired methicillin resistant Staphylococcus aureus (MRSA) has dramatically eroded the efficacy of current therapeutics. We describe a chemical genetic strategy using antisense interference to broadly identify new drug targets that potentiate the effects of existing antibiotics against both etiological classes of MRSA infection. Further, ...

OBJECTIVE Skin testing with penicilloyl-polylysine (PPL) and a minor determinant mixture (MDM) were previously recommended for evaluating β-lactam hypersensitivity. However, PPL and MDM have not been commercially available. This study was to determine the negative predictive value (NPV) of skin testing with β-lactam antibiotics for the diagnosis of β-lactam hypersensitivity. METHOD Patients a...

Background A high frequency of hypersensitivity reactions to -lactam antibiotics are observed in patients with cystic fibrosis. -lactam antibiotics form protein conjugates in vitro and in vivo, and a core group of lysine residues of human serum albumin have been shown to be penicilloylated by mass spectrometric methods. Furthermore, protein/ peptide conjugates have been shown to stimulate T cel...

PURPOSE In gram-negative bacteria, active efflux pumps that excrete drugs can confer resistance to antibiotics however, in Helicobacter pylori this role is not well established. The purpose of this study is to evaluate the role of active efflux in resistance of H. pylori isolates to antibiotics. MATERIALS AND METHODS Twelve multiple antibiotic resistant (MAR) isolates resistant to at least fo...

Violet-pigmented bacteria, which have been described since the end of the 19th century, are occasionally the causative agent of septicemia and sometimes cause fatal infection in human and animals. Bacteria, producing violet colonies due to the production of a nondiffusible pigment violacein, were classified as a redefined genus Chromobacterium. Chromobacterium violaceum is gram-negative, and sa...

BACKGROUND AND OBJECTIVES Carbapenems are beta-lactam antibiotics, presently considered as most potent agents for treating multi-drug resistant Gram-negative bacilli infections. In India carbapenems available for use are meropenem and imipenem, introduced recently. Resistance to these has been reported in a few bacteria especially Pseudomonas spp. We therefore retrospectively evaluated the anti...

Aminoglycosides (AGs) are often used in combination with -lactam antibiotics for the treatment of serious Gram-negative infections. Traditionally, AGs have been dosed multiple times a day. Over the past decade, extended-interval dosing has been evaluated as a method of improving the efficacy and safety of AGs. Extended-interval aminoglycoside dosing (EIAD) generally consists of administering th...

The properties of cefazolin, cefoxitin, cefamandole, and cefuroxime have been compared with those of cephaloridine, cephalothin, and cephalexin. The properties included for examination are the susceptibility to a range of beta-lactamases commonly encountered in gram-negative species and the ability of a beta-lactam antibiotic to penetrate the outer layers of Escherichia coli. Of the antibiotics...

As a novel strategy to remove β-lactam antibiotic residues from fish tissues, utilization of β-lactamase, enzyme that normally degrades β-lactam structure-containing drugs, was explored. The enzyme (TEM-52) selectively degraded β-lactam antibiotics but was completely inactive against tetracycline-, quinolone-, macrolide-, or aminoglycosidestructured antibacterials. After simultaneous administra...