New Delhi metallo-β-lactamase (NDM-1) is a new metallo-β-lactamase (MBL) that has recently emerged as a global threat because it confers bacteria with resistance to almost all clinically used β-lactam antibiotics. To determine the molecular basis of this threat, NDM-1 was purified from Escherichia coli TransB (DE3) carrying cloned blaNDM-1 gene by an anion-exchange chromatography step followed ...

Resistance to 3rd generation cephalosporins in Salmonella has been attributed to the production of extended spectrum β-lactamases (ESBLs), but AmpC and KPC or metallo β-lactamase (MBL) mediated resistance have also been reported1. ESBLs reported in Salmonella spp. are the derivatives of TEM, SHV, CTX-M and PER type β-lactamase families1. We isolated Salmonella enterica ser. Typhimurium (B9903) ...

metallo-β-lactamase (mbl) producing pseudomonas aeruginosa has been reported to be an important nosocomial infection. its intrinsic and acquired resistance to various antimicrobial agents and its ability to develop multidrug resistance imposes a serious therapeutic problem. different clinical samples were collected from public and private hospitals in baghdad city, iraq. bacterial identificatio...

Urinary tract infections (UTIs) are among the most common diagnosed in clinical practice. Treatment is often initiated before microbiological confirmation and anti-microbial susceptibility testing. With rapidly rising antibiotic resistance treatment failures not uncommon. Beta-lactamase production by gram-negative bacteria causing UTI commonest mode of drug resistance. The aim current study was...

BACKGROUND One of the major clinical problems regarding Pseudomonas aeruginosa is attributed to metallo-beta-lactamases (MBL). This group of enzymes is a subset of beta lactamases which belong to group B of Ambler classification and cause hydrolysis of carbapenems. Based on epidemiological studies conducted worldwide, it is proved that prevalence of genes coding MBLs in P. aeruginosa species ar...

BACKGROUND Metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa is a significant pathogen in burn patients. OBJECTIVES The aim of this study was to determine the prevalence of carbapenem-resistant P. aeruginosa isolates, including those resistant to imipenemase (IMP), in a burn unit in Isfahan, Iran. PATIENTS AND METHODS One hundred and fifty P. aeruginosa isolates from burn patients ...

OBJECTIVES To investigate the mechanisms of carbapenem resistance in the 175 Pseudomonas aeruginosa isolates (39%; 175/448) showing non-susceptibility (European Committee on Antimicrobial Susceptibility Testing breakpoints) to imipenem (35%), meropenem (33%) and/or doripenem (33%) recovered in 2008-09 from 16 Spanish hospitals during the Comparative Activity of Carbapenem Testing (COMPACT) surv...

BACKGROUND Carbapenem resistant Acinetobacter baumannii is an important nosocomial pathogen associated with a variety of infections. OBJECTIVES The current study aimed to characterize the antimicrobial susceptibility, analyze the prevalence of oxacillinase and metallo-β-lactamase (MBL) genes and molecular typing of clinical isolates of A. baumannii. MATERIALS AND METHODS A total of 124 non-...

conclusions the rate of carbapenem resistant a. baumannii isolates were high in this study. since, blaoxa-58-like or mbl genes were not detected, it seems that resistance to carbapenems is related to blaoxa-23-like and blaoxa-24-like. moreover, blaoxa-23-like was the most prevalent oxacillinase (oxa) gene. most of the isolates belonged to one of the four dominant genotypes indicating clonal dis...