Background and Objective: Organisms producing CTX-M β-lactamases are emerging as a source of resistance to oxyiminocefalosporins such as ceftriaxone and ceftazidime. However, the laboratory detection of these strains is not well defined. In this study, phenotypic assay for screening of extended-spectrum β-lactamases producing strains and molecular assay for the identification of CTX-M β-lact...

The main objective is to study the genotypic and characterization of plasmid deoxyribonucleic acid content resistant isolates in Erbil city. A total 39 Acinetobacter baumannii were isolated from 102 diabetic foot ulcer patients, whose ages ranged 37 88 y, attending various medical wards hospitals city, Iraq, between May 2020 March 2021. Isolates tested for presence extended-spectrum beta-lactam...

BACKGROUND The emergence of carbapenem-resistant Klebsiella pneumoniae poses a serious problem to antibiotic management. We investigated the β-lactamases in a group of carbapenem-resistant K. pneumoniae clinical isolates from Turkey. METHODS Thirty-seven strains of K. pneumoniae isolated from various clinical specimens were analyzed by antimicrobial susceptibility testing, PCR for the detecti...

Escherichia coli is one of the leading causes of nosocomial infections. The most frequent mechanism of resistance to penicillins is the production of the common plasmidmediated TEM-1 -lactamase. Extended-spectrum lactamases of the TEM and SHV families, conferring resistance to newer -lactams such as oxyimino-cephalosporins, have also spread among E. coli clinical isolates. These enzymes are una...

BACKGROUND Despite increasing importance of Acinetobacter baumannii in nosocomial infections and rapid development of multi-antimicrobial resistance in this strain, the resistance mechanisms of beta-lactam antimicrobials in A. baumannii were not clearly defined. In order to observe the resistance mechanisms against beta-lactams and carbapenem, we characterized the production of beta-lactamases ...

This study reports the occurrence of antibiotic resistance and production of β-lactamases including extended spectrum beta-lactamases (ESβL) in enteric bacteria isolated from hospital wastewater. Among sixty-nine isolates, tested for antibiotic sensitivity, 73.9% strains were resistant to ampicillin followed by nalidixic acid (72.5%), penicillin (63.8%), co-trimoxazole (55.1%), norfloxacin (53....

The in vivo activity and source of beta-lactamase in sputum samples from 43 patients with cystic fibrosis (CF) during a 2-week antipseudomonal treatment were studied. A colorimetric method, based on the conversion of nitrocefin, was used for quantitation of the sputum beta-lactamase activity. beta-Lactamases in sputum were characterized by isoelectric focusing and inhibition profile and were co...