One of the major constraints facing laser speckle imaging for blood-flow measurement is reliable measurement of the correlation time (τ(C)) of the back-scattered light and, hence, the blood's speed in blood vessels. In this Letter, we present a new model expression for integrated speckle contrast, which accounts not only for temporal integration but spatial integration, too, due to the finite s...

We build a photoacoustic imaging system using an intensity-modulated continuous-wave laser source, which is an inexpensive, compact, and durable 120-mW laser diode. The goal is to significantly reduce the costs and sizes of photoacoustic imaging systems. By using a bowl-shaped piezoelectric transducer, whose numerical aperture is 0.85 and resonance frequency is 2.45 MHz, we image biological tis...

We report the compositional engineering of silicongermanium planar microstructures through laser processing. The effects of the laser treatment are assessed through microscope imaging and Raman spectroscopy. Our results reveal that the laser-exposed regions display a significant change in the material

A CCD imaging system has been developed for precision laser beam pointing. The systcm contains a high frame rate CCD camera operated in windowing mode under command of an ernbeddcd processor. A ltiser pointing and tracking update rate of 2 KIIz has been achieved. Subject terms: CCD cameras, laser pointing, laser communications.

Although studies have shown that photothermal therapy can coagulate selectively abnormal vasculature, the ability of this method to achieve consistent, complete removal of the vasculature is questionable. We present the use of multimodal, wide-field functional imaging to study, in greater detail, the biological response to selective laser injury. Specifically, a single-platform instrument capab...

A customized laser combiner was designed and constructed for dual channel single molecule imaging. The feasibility of a combiner-incorporated imaging system was demonstrated in studies of single molecule FRET. Distance rulers made of dual-labeled dsDNA were used to evaluate the system by determining the distance between one FRET pair. The results showed that the system is sensitive enough to di...

This review article discusses the use of laser Doppler imaging as a clinimetric tool to determine burn depth in patients presenting to hospital. Laser Doppler imaging is a very sensitive and specific tool to measure burn depth, easy to use, reliable and acceptable to the patient due to its quick and non-invasive nature. Improvements in validity, cost and reproducibility would improve its use in...

A Fourier domain mode-locked (FDML) laser at 1050 nm for ultra-high-speed optical coherence tomography (OCT) imaging of the human retina is demonstrated. Achievable performance, physical limitations, design rules, and scaling principles for FDML operation and component choice in this wavelength range are discussed. The fiber-based FDML laser operates at a sweep rate of 236 kHz over a 63 nm tuni...

The application of second-harmonic generation (SHG) microscopy to plant materials has been neglected hitherto even though it would seem to have promise for identification and characterization of biologically and commercially important plant polysaccharides. We find that imaging of cellulose requires rather high laser powers, which are above optimal values for live cell imaging. Starch, however,...

We report the design and implementation of a high-speed, automated laser radar (LADAR) system with sensitivity enhanced by polarimetric imaging technique. This LADAR is able to analyze the Stokes vector of the reflected light from a target at video-rate. With a polarization state generator and a polarization state analyzer, the system is capable of performing a complete Mueller matrix imaging o...