The unique goal of the Living Computer Museum (LCM) in Seattle, Washington, is to keep old computer technology powered up and ready to be used by patrons. Instead of sheltering its rare arti facts in climate-controlled glass cases, most of the computers at the LCM are turned on and accompanied by a user manual to read, a chair to sit in, and a keyboard to type on so you can explore what it was ...

An immunohistochemical technique was developed to visualize nitric oxide synthase (NOS)-immunopositive neurons in fresh-frozen tissue sections of rat brain for laser capture microdissection (LCM) and mRNA analysis. The effect of tissue fixation and the choice of fluorophore were investigated. Here we describe a rapid immunofluorescence protocol that allows the processing of fresh-frozen tissue ...

DNA methylation is a mechanism for long-term transcriptional regulation and is required for normal cellular differentiation. Failure to properly establish or maintain DNA methylation patterns leads to cell dysfunction and diseases such as cancer. Identifying DNA methylation signatures in complex tissues can be challenging owing to inaccurate cell enrichment methods and low DNA yields. We have d...

Laser capture microdissection (LCM) is a powerful tool that enables the isolation of specific cell types from tissue sections, overcoming the problem of tissue heterogeneity and contamination. This study combined the LCM with isotope-coded affinity tag (ICAT) technology and two-dimensional liquid chromatography to investigate the qualitative and quantitative proteomes of hepatocellular carcinom...

Land cover mapping (LCM) in complex surface-mined and agricultural landscapes could contribute greatly to regulating mine exploitation and protecting mine geo-environments. However, there are some special and spectrally similar land covers in these landscapes which increase the difficulty in LCM when employing high spatial resolution images. There is currently no research on these mixed complex...

BACKGROUND The intestinal mucosa is the compartment that sustains the most severe injury in response to radiation and is therefore of primary interest. The use of whole gut extracts for analysis of gene expression may confound important changes in the mucosa. On the other hand, laser capture microdissection (LCM) is hampered by the unstable nature of RNA and by a more complicated collection pro...

The intestinal ecosystem is formed by a complex, yet highly characteristic microbial community. The parameters defining whether this community permits invasion of a new bacterial species are unclear. In particular, inhibition of enteropathogen infection by the gut microbiota ( = colonization resistance) is poorly understood. To analyze the mechanisms of microbiota-mediated protection from Salmo...

Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes preferential lysis of epithelial contaminants followed by collection of intact sperm by centrifugation. While effective for samples where sperm ar...

We introduce topographic versions of two latent class models (LCM) for collaborative filtering. Latent classes are topologically organized on a square grid. Topographic organization of latent classes makes orientation in rating/preference patterns captured by the latent classes easier and more systematic. The variation in film rating patterns is modelled by multinomial and binomial distribution...

In this paper we primarily study monomial ideals and their minimal free resolutions by studying their associated lcm-lattices. In particular, we formally define the notion of coordinatizing a finite atomic lattice P to produce a monomial ideal whose lcm-lattice is P , and we give a characterization of all such coordinatizations. We prove that all relations in the lattice L(n) of all finite atom...