A colorimetric method is described for the measurement of serum lactic dehydrogenase (LDH) activity. The method employs phenazine methosulfate to mediate electron transfer between reduced diphosphopyridinenucleotide and 2,6-dichloroindophenol,and allows continuous measurement of the reaction rate in the direction lactate to pyruvate. The LDH of a number of normal and pathologic sera was measure...

Citation: Cho C-H (2015) Commentary: Targeting LDH enzymes with a stiripentol analog to treat epilepsy. Meeting energy demands in neurons is critical for proper functions of nervous systems. In addition to glucose, lactate is used as a major energy source in the brain, and a significant amount of lactate is produced through aerobic glycolysis in astrocytes It is reversibly converted to and from...

BACKGROUND Lactate formation is up-regulated in tumorous cells by lactate dehydrogenase (LDH). High serum LDH level is linked to many malignancies with poorer survival, but tumour LDH-5 has not been well investigated in non-small cell lung cancer (NSCLC). PATIENTS AND METHODS In 89 patients operated on for NSCLC stage I-III, the serum LDH level was assayed and immunohistochemistry for tumour ...

Crystals of lactate dehydrogenase (LDH) were frozen by equilibration in a sucrose-ammonium sulfate solution, and then dipping into liquid nitrogen. The rate of radiation damage for frozen crystals was tenfold less than for crystals at room temperature. The physical properties of frozen crystals are discussed. Analysis of 3"5 A data collected at -75°C for native LDH and two heavy atom derivative...

Examensarbete 16 hp Lactate Dehydrogenase and Citrate Synthase activity in cardiac and skeletal muscle of lowland and highland tinamous Naomi Aira Lactate Dehydrogenase and Citrate Synthase activity in cardiac and skeletal muscle of lowland and highland tinamous. Sammanfattning/Abstract: Tinamous (Tinamidae) have the smallest heart in relation to body mass compared to any other flying bird toda...

In recent studies the lactate dehydrogenase (LDH) isozymes of adult (Lindsay, '63) and embryonic (Philip and Vesell, '62; Nebel and Conklin, '64) chick liver were demonstrated. A maximum of six isozymes were found in the embryonic liver while four isozymes were present in the liver after hatching. While several theories (Apella and Markert, '61; Markert, '62; Cahn et al., '62; Fritz and Jacobso...

Lactate dehydrogenase (LDH) X has been isolated in crystalline form from mouse testes. Homogeneity of the protein preparation was established by disc gel electrophoresis, analytical ultracentrifugation, and immunochemical analyses. LDH-X is clearly distinct in amino acid composition from LDH-1 and LDH-5 with respect to numbers of residues of leucine, glycine, threonine, and methionine. When 6 t...

Lactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase level of the lactic acid and lactic acid salts. The objective of this investigation is establishing ...

Serum and tumor tissue of a patient with neuroblastoma contained an abnormal isoenzyme of lactate dehydrogenase (LDH; EC 1.1.1.27), which, on agarose gel electrophoresis, migrated between LDH-2 and LDH-3 with a mobility the same as that of the extra LDH isoenzyme found in normal human erythrocytes. On surgical removal of the tumor, the high total LDH activity (775 U/L) in the serum of the patie...

Lactate dehydrogenase (LDH) X has been isolated in crystalline form from mouse testes. Homogeneity of the protein preparation was established by disc gel electrophoresis, analytical ultracentrifugation, and immunochemical analyses. LDH-X is clearly distinct in amino acid composition from LDH-1 and LDH-5 with respect to numbers of residues of leucine, glycine, threonine, and methionine. When 6 t...