BACKGROUND Leptospirosis is a worldwide zoonosis that is endemic in tropical areas, such as Reunion Island. The species Leptospira interrogans is the primary agent in human infections, but other pathogenic species, such as L. kirschner and L. borgpetersenii, are also associated with human leptospirosis. METHODS AND FINDINGS In this study, a melting curve analysis of the products that were amp...

Background Leptospirosis is a zoonotic disease of worldwide distribution that affects humans and domestic wild animals, it highly endemic in Ecuador. However, no reports infections affecting horses have been published the country. Methods This study evaluates prevalence anti- Leptospira spp. antibodies racing from breeding farm coastal Santa Elena province, southwest Sera were collected 108 non...

OBJECTIVE To detect leptospiral antibodies by microscopic agglutination test (MAT) in north-east of Iran. METHODS This study was conducted to evaluate prevalence of human leptospiral infections by MAT, using six current reference strains of Leptospira interrogans in north-east of Iran. A total of 285 serum samples were collected from three north-east provinces of Iran, from December, 2009 to ...

Leptospirosis is a worldwide distributed zoonosis, caused by pathogenic strains of spirochetes belonging to the genus Leptospira spp. So far, the only gold standard diagnostic method for this disease has been the Micro-Agglutination Test (MAT). The purpose of this study was to test whether a correlation exists between serological diagnosis by MAT and the presence of Leptospira spp. DNA traces i...

AbstractsObjective This is the first study was conducted to identify Leptospirosis seroprevalence and serovar distribution among healthy people in different regions Vietnam. Methods cross-sectional with 600 asymptomatic three provinces, representing for North, northern Central, South of Vietnam from November December 2019. Each participant collected a 3 ml venous bloo...

Genetic maps were constructed for Leptospira interrogans serovars icterohaemorrhagiae and pomona. Previously we independently constructed physical maps of the genomes for these two serovars. The genomes of both serovars consist of a large replicon (4.4 to 4.6 Mb) and a small replicon (350 kb). Genes were localized on the physical maps by using Southern blot analysis with specific probes. Among ...

Leptospirosis is one of the zoonotic diseases caused by different serovars of the species Leptospira interrogans.  The source of infection in humans is usually either direct or indirect contact with the urine of infected animals.  In this study, 400 human blood samples collected from tribal areas of Farsan and Koohrang cities in West Centeral Iran.  The serum samples were analyzed employing Mic...

leptospirosis is a zoonotic disease with global distribution that caused by pathogenic spirochetes of the genus leptospira. accurate diagnosis for differentiation of leptospirosis from other pyrogenic infections prevailing in the same locality and is imperative for proper treatment. therefore a molecular diagnostic test with high specificity and sensitivity such as pcr is essential. gene encodi...

The genomic structures of spirochete species are not well characterized, and genetic studies on these organisms have been hampered by lack of a genetic exchange mechanism in these bacteria. In view of these observations, pulsed-field gel electrophoresis was used to examine the genomes of Leptospira species. Live cells, prepared in agarose plugs, were lysed in situ, and the DNA was analyzed unde...

Leptospirosis is a zoonosis, which has become serious problem, especially in areas with high rainfall. Massive leptospirosis occurs after heavy rains or floods. This study aims to analyze the types and distribution of leptospira bacteria serovars risk factors for post-flood dogs. The research was conducted from January March 2020 at Ragunan Animal Hospital independent clinics Jakarta, Bekasi, D...