Depeneding on the quality and quantity of herbicides and their mode of action and soil type, the number of different groups of microorganisms in soil increased or decreased. Sulphonylurea herbicides belong to a group of pesticides which in doses of only a few grams per hectar are sufficient to control weeds. The mode of action of these herbicides is based on blocking the enzyme acetolactate syn...

ABSTRACT Sweat is produced by the apocrine glands, if infected bacteria that play a role in decay process will certainly produce foot odor. Some of cause, including Staphylococcus epidermis, Corynebacterium acne and there one bacterium causes pungent odor Bacillus subtilis. subtilis enzyme leucine dehydrogenase highest, resulting isovaleric acid Lime peel (Citrus aurantifolia) has potential to ...

very recently, genes whose effects are described at the protein level uNzv! been unknown in Drosophila. Gel electrophoresis, however, has remedied this situation. Within the past few years, the following gene-enzyme systems have been elucidated by means of this technique: Amylase (KIKKAWA 1964), Esterase 6 (WRIGHT 1963), Esterase C (BECKMAN and JOHNSON 1964a), Glucose-6-phosphate dehydrogenase ...

Acute neurological crises involving striatal degeneration induced by a deficiency of glutaryl-CoA dehydrogenase (GCDH) and the accumulation of glutaric (GA) and 3-hydroxyglutaric acid (3-OHGA) are considered to be the most striking features of glutaric aciduria type I (GA1). In the present study, we investigated the mechanisms of apoptosis and energy metabolism impairment in our novel GA1 neuro...

1. The effect of the branched-chain amino acids, namely leucine, isoleucine and valine and their corresponding 2-oxo acids on the metabolism of 2-oxoglutarate by developing rat and human brain preparations was investigated. 2. The decarboxylation of 2-oxo[1-(14)C]glutarate to (14)CO(2) by mitochondria from adult rat brain was inhibited by the branched-chain 2-oxo acids whereas the branched-chai...

We previously demonstrated efficient L-valine production by metabolically engineered Corynebacterium glutamicum under oxygen deprivation. To achieve the high productivity, a NADH/NADPH cofactor imbalance during the synthesis of l-valine was overcome by engineering NAD-preferring mutant acetohydroxy acid isomeroreductase (AHAIR) and using NAD-specific leucine dehydrogenase from Lysinibacillus sp...

The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in Mucor circinelloides. This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in M. circinelloides. To clarify this, the...

Micrococcus luteus naturally produces alkenes, unsaturated aliphatic hydrocarbons, and represents a promising host to produce hydrocarbons as constituents of biofuels and lubricants. In this work, we identify the genes for key enzymes of the branched-chain amino acid catabolism in M. luteus, whose first metabolic steps lead also to the formation of primer molecules for branched-chain fatty acid...

Using cultured skin fibroblasts, we studied the heterogeneity of inborn errors of leucine metabolism such as isovaleric acidemia (IVA), glutaric aciduria type II (GA II), and multiple carboxylase deficiency (MC). We first developed a simple macromolecular-labeling test to measure the ability of cells to oxidize [1-14C]isovaleric acid in situ in culture. Cells from two different lines were fused...