A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79 g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor ...

Two-dimensional gel electrophoretic analysis of cell lysates from Brucella abortus 2308 and the isogenic hfq mutant Hfq3 revealed that the RNA binding protein Hfq (also known as host factor I or HF-I) is required for the optimal stationary phase production of the periplasmic Cu,Zn superoxide dismutase SodC. An isogenic sodC mutant, designated MEK2, was constructed from B. abortus 2308 by gene r...

Plectranthus spp (Lamiaceae) are plants of economic importance because they are sources of aromatic essential oils and are also cultivated and several species of this genus are used as folk medicines. This paper describes the effects of different concentrations of the 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthaleneacetic acid (NAA) on the induction of callus from nodal segments of Plec...

the genus tanacetum l. belongs to the asteraceae family and anthemideae tribe is one of the most important medicinal plants that contains 26 species in iran, 12 of them are endemic. this paper reports the essential oil composition of tanacetum uniflorum (fisch. & c.a. mey. ex dc.) sch.bip. growing spontaneously in salmas (north-west of iran). essentail oil extracted by water distillation and st...

The essential oil obtained by hydrodistillation of flowers of Calycanthus floridus L. var.oblongifolius (Nutt.) D.E. Boufford & S.A. Spongberg, planted in Sabzevar, Khorasan Province(Iran), was analyzed by GC and GC/MS. Eighteen compounds representing 90.1% of theflower oil of Calycanthus floridus L. var. oblongifolius were identified, among them 1,8-cineol(33.1%), bornyl acetate (14.1%), α-pin...

The study was conducted to determine the insecticidal and behavioral effect of essential oils (EOs) extracted from three lavender cultivars, Hemus, Raya Yubileina, against adult stage Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae) under laboratory conditions. contact, fumigant repellent effects plant EOs were investigated possible germination inhibitory on wheat evaluated. samples ...

introduction: there have been great efforts to find safe and potent natural antioxidants from various plant sources. there is, at present, increasing interest both in the industry and scientific research for spices and aromatic herbs because of their strong antioxidant and antimicrobial properties, which exceed many currently used natural and synthetic antioxidants. medicinal plants are complex...

A stereoselective synthesis of 1-substituted (E)-2-aryl-but-1-en-3-ynes, including tetrasubstituted alkenes, has been developed from aryl-substituted α-allenols by treatment with the AcCl-NaOH (aqueous) system. This transformation might be explained through the elimination of acetic acid, made up of a δ-hydrogen and the acetate group in the initially formed α-allenic acetate.

Diabetes mellitus is a multifactorial metabolic disease characterized by post-prandial hyperglycemia (PPHG). α-amylase and α-glucosidase inhibitors aim to explore novel therapeutic agents. Herein we report the promises of Dioscorea bulbifera and its bioactive principle, diosgenin as novel α-amylase and α-glucosidase inhibitor. Among petroleum ether, ethyl acetate, methanol and 70% ethanol (v/v)...