We describe a novel activatable probe for fluorine-19 NMR based on self-assembling amphiphilic dendrons. The dendron probe has been designed to be spectroscopically silent due to the formation of large aggregates. Upon exposure to the specific target enzyme, the aggregates disassemble to give rise to a sharp 19F NMR signal. The probe is capable of detecting enzyme concentrations in the low nano...

The aim of present study was to compare the diagnostic performance of the different Brucella abortus antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR ...

Lipases are the most widely employed enzymes in commercial industries. The catalytic mechanism of most lipases involves a step called "interfacial activation". As interfacial activation can lead to a significant increase in catalytic activity, it is of profound importance in developing lipase immobilization methods. To obtain a potential biocatalyst for industrial biodiesel production, an effec...

The neuropathological hallmark of Parkinson’s disease (PD) is the accumulation alpha–synuclein (AS) aggregates. identification AS aggregates in gut biopsy specimens from people with PD may provide an opportunity to identify at a very early stage, prior symptom onset. Changes microbiota and inflammatory conditions (such as periodontitis) be linked onset/evolution. This project aims explore conce...

In this tutorial review, an overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented. The importance of biocatalysis in the context of green and sustainable chemicals manufacture is discussed and the necessity for immobilisation of enzymes as a key enabling technology for practical and commercial viability is emphasised. The underlying reasons for immobili...

Biophysical characterisation of type A botulinum neurotoxin (BoNT/A) could be challenging since it exists in association with associated proteins (NAPs) as large protein complexes. The objective this study was to elucidate the dissociation mechanics BoNT/A complex along its thermodynamic stability through a combination analytical tools. Size exclusion chromatography (SEC) mainly used behavior a...