A lipolytic enzyme has been demonstrated by means of a cytochemical technique and by direct chemical assay in granulomatous tissues of guinea pigs with encephalomyelitis and demyelinization resulting from the injection of an emulsion comprised of brain tissue and adjuvants, including Mycobacterium butyricum. Combined histologic and cytochemical studies showed that the lipolytic enzyme was prese...

The effects of Bacillus subtilis protease, Type VIII (subtilopeptidase A, EC 3.4.4.16), on the lipolytic action of norepinephrine, corticotropin, caifeine, and theophylline on isolated adipose cells were examined. The results indicate that B. subfilis protease, at concentrations suboptimal and optimal for the enhancement of glucose utilization, inhibited, like insulin, the lipolysis mediated by...

Lipolytic enzymes, esterases (EC 3.1.1.1) and lipases (EC 3.1.1.3), catalyze the hydrolysis of ester bonds between alcohols and carboxylic acids, and its formation in organic media. At present, they represent about 20% of commercialized enzymes for industrial use. Lipolytic enzymes from thermophilic microorganisms are preferred for industrial use to their mesophilic counterparts, mainly due to ...

An indirect method for obtaining a reliable measure of the rate of glucose transport into adipocytes is described. Evidence is presented that altered levels of 3':5'-cyclic AMP can influence the transport of glucose into adipocytes. When cyclic AMP levels were lowered with antilipolytic agents (insulin, nicotinic acid, or clofibrate), rates of glucose transport were increased. In contrast, when...

The effect of Vespa amino acid mixture (VAAM) on the release of lipolytic products was examined in isolated rat adipocytes. Concentrations of 112.5 to 225 ppm of VAAM showed significantly greater release of non-esterified fatty acids (NEFA) and glycerol than the same concentrations of casein amino acid mixture (CAAM). The integrated relative release of NEFA and glycerol was lower in response to...

Cholera toxin, in contrast to its stimulatory action on adenylate cyclase, renders the insulin-stimulated adipose cell insensitive to the inhibitory actions of lipolytic agents such as isoprenaline, adrenocorticotropic hormone or glucagon. Conversely, pertussis toxin eliminates both the stirnulatory actions of the antilipolytic agents, such as adenosine, nicotinic acid, and prostaglandin E , , ...

1. Adipocytes isolated from rats 6-9 days after adrenalectomy had significantly increased sensitivity to insulin action against noradrenaline-stimulated lipolysis. In the presence of adenosine deaminase there was no significant difference in insulin sensitivity between cells from adrenalectomized and sham-operated rats. 2. Adipocytes from adrenalectomized rats had decreased lipolytic responses ...

Summary Production of Thai fish paste Ka‐pi‐plaa fermented using beardless barb, Cyclocheilichthys apogon was monitored. The physicochemical, microbiological and antioxidant properties were compared after each process, that is autolysis, salting, sun‐drying fermentation. Colour parameters L* decreased while a* b* increased during production ( P < 0.05). finished product presented an intense ...