The combination of HPLC and mass spectrometry has great promise for the toxicology laboratory. In the past five years, significant progress has been made toward producing a reliable interface for these techniques. Thermospray, liquid ion evaporation, ion-spray, and the particle beam separator are all viable "second generation" approaches with significant advantages and disadvantages. I review t...

A new impurity was detected during high performance liquid chromatographic (HPLC) analysis of eslicarbazepine acetate active pharmaceutical ingredient. The structure of unknown impurity was postulated based on liquid chromatography mass spectrometry using electrospray ionization and ion trap analyzer (LC/ESI-IT/MS) analysis. Proposed structure of impurity was unambiguously confirmed by synthesi...

1 Abbreviations used: [methyl-C]AdoMet, S-adenosyl-L-[methyl-C]methionine; [methyl-H]AdoMet, S-adenosyl-L-[methyl-H]methionine; aDMA, asymmetric x-N,N-dimethylarginine; AdoMet, S-adenosyl-L-methionine; AdoHcy, S-adenosylL-homocysteine; CID, collision-induced dissociation; DMA, dimethylarginine; DTT, dithiothreitol; ESI, electrospray ionization; ETD, electron-transfer dissociation; FPLC, fast pe...

The high performance liquid chromatography-electrospray ionization-mass spectrometry method has been applied to the measurement of bile acid transport activities in membrane vesicles obtained from a human bile salt export pump expressing Sf9 cells. The amounts of bile acids transported using the human bile salt export pump expressing Sf9 cells were determined using liquid chromatography-electro...

A versatile microreactor protocol based on microfluidic droplets has been developed for on-line protein digestion. Proteins separated by liquid chromatography are fractionated in water-in-oil droplets and digested in sequence. The microfluidic reactor acts also as an electrospray ionization emitter for mass spectrometry analysis of the peptides produced in the individual droplets. Each droplet ...

Arapid, highly sensitive and efficient flotation/magnetic stirring-assisted liquid-liquid microextraction combined with flame atomic absorption spectrometry has been proposed for the preconcentration and quantitative analysis of trace amounts of cadmium (II) in the presence of sodium diethyldithiocarbamateas complexingagent. Parameters including sample pH, concentration of the complexingagent, ...

Applications, features, and mechanistic details of laser assisted liquid phase dispersion mass spectrometry are highlighted and discussed. It has been used in the past to directly isolate charged molecular aggregates from the liquid phase and to determine their molecular weight employing sensitive time-of-flight mass spectrometry. The liquid matrix in this MALDI (matrix assisted laser desorptio...

A liquid chromatography/mass spectrometry (LC/MS) method using desorption electrospray ionization (DESI) as a versatile interface has been established, which allows a wide range of elution flow rates, online derivatization via reactive DESI and further combination with electrochemistry.