background: non-alcoholic fatty liver disease (nafld) is increasingly a common disease which is created by the accumulation of fat in the liver. about 20% of adults and 5% of children suffer from this disease. in some studies, half of obese children had fatty liver disease and approximately 90% of the patients with fatty liver were obese. the aim of this study was to evaluate fatty liver diseas...

Previous biochemical studies have indicated that the membrane-bound thyrotropin-releasing hormone (TRH)-degrading enzyme (TRH-DE) from brain and liver and the serum TRH-DE are derived from the same gene. These studies also suggested that the serum enzyme is of liver origin. The present study was undertaken to verify these hypotheses. In different species, a close relationship between the activi...

crocin, a bioactive molecule of saffron can be purely isolated from the saffron extract. it has different pharmacological effects such as antioxidant and anticancer activities. morphine is an opioid analgesic drug. it is mainly metabolized in liver and causes devastating effects. it can increase the generation of free radicals. this study was designed to evaluate the protective role of crocin a...

In this study, the glutathione S-transferase enzyme (GST; EC 2.5.1.18) was purified with 8.35 EU/mL specific activity, 24.56 times 8% yield, from chicken liver, using ammonium sulfate precipitation and glutathione-agarose affinity chromatography. order to control purity of enzyme, SDS-PAGE performed a single band obtained. The molecular mass subunit calculated as approximately 30.9 kDa. additio...

The enzyme alcohol dehydrogenase (ADH) is a dimeric in which each of its subunits has Zn2+ metal-containing catalytic domain and cofactor binding domain. This converts into an aldehyde. In this article, the activity was investigated by applying immobilization process directly to purified activated florisil from chicken liver. For purpose, homogenization liver achieved supernatants were separate...

Squalene epoxidase activity has been studied in cell-free preparations of Chinese hamster ovary (CHO) cells and rat liver. In contrast to rat liver microsomal squalene epoxidase, the enzyme of CHO cells is only slightly activated by the autologous cytosolic fraction, whereas phosphatidylglycerol or rat liver cytosolic preparations are potent stimulators of this enzyme. Triton X-100, a known sti...

Pyruvate carboxylase (PC) has been quantified in rat liver and kidney cortex under experimental conditions that modify the gluconeogenic response in both organs: fasting, carbon tetrachloride-induced liver degeneration and alloxan-induced diabetes. Enzymatic activity has been assayed by a 14CO2-fixation method. The amount of enzyme has been determined by competitive ELISA using antibodies raise...

Sorbitol dehydrogenase (SDH) is involved in the polyol pathway, which plays an important role in the pathogenesis of diabetic complications. We have measured the tissue distributions of SDH mRNA, both the immunoreactive enzyme levels and the enzyme activity. SDH mRNA was especially abundant in liver, kidney and testis. Both the activity and enzyme content are high in liver and kidney but not in...

delta-Aminolaevulinate dehydratase activity was measured in liver biopsy specimens from 13 patients with porphyria cutanea tarda, 13 alcoholics and 10 control subjects. The mean enzyme activities in liver of porphyria cutanea tarda and alcoholics were 34% (P less than 0.001) and 49% (P less than 0.001) respectively of the control level. Heat treatment of liver supernatant caused slight inhibiti...

AIMS/INTRODUCTION We sought to determine the association between change in fasting plasma glucose (FPG) and levels of liver enzymes, such as aspartate transaminase, alanine transaminase and gamma-glutamyltransferase, from health examinations. MATERIALS AND METHODS A total of 9,393 health screen examinees with no evidence of viral hepatitis, liver diseases, abnormal liver function and diabetes...