The influence of the amount of heparin injected on the contributions of liver and of extrahepatic tissues to the lipase activity of postheparin serum of the rat was studied. It was found that when high doses of heparin (20 I.U./lOO g bodyweight) were injected, the liver contributes for 63% to the total triacylglycerol lipase activity of postheparin serum. At low heparin levels (0.4 I.U./lOO g b...

Purified bovine heart 6-phosphofructo-2-kinase can be phosphorylated in the presence of protein kinase C and dephosphorylated by alkaline phosphatase; changes in phosphorylation state have no effect on enzyme activity. By contrast, the rat liver enzyme is a poor substrate for protein kinase C. Unlike the liver enzyme, which is bifunctional and is phosphorylated by fructose 2,6-[2-32P]bisphospha...

We employed a newly developed radioimmunoassay to quantitate P-enolpyruvate carboxykinase protein directly in liver and kidney of intact rats. The radioimmunoassay was dependent upon the presence of sodium dodecyl sulfate in the competitive binding reaction mixture. In conjunction with assayable activity measurements, the radioimmunoassay results also made it possible to assess the average spec...

background: tyrosinemia type 1 is an autosomal recessive metabolic disorder, which typically affects liver and kidneys. it is caused by a defect in fumarylacetoacetate hydrolase or fumarylacetoacetase (fah) enzyme, the final enzyme in the tyrosine degradation pathway. the disease typically manifests as early onset type in early infancy with acute hepatic crisis with hepatomegaly and bleeding te...

thyrotoxicosis is a condition in which tissues are stimulated by increased secretion of thyroid hormone. the most common cause is diffuse toxic goiter and toxic multi-nodular goiter. for more reviews on this disease, the effects of hyperthyroidism on liver enzyme levels were studied. a total of 30 adult male wistar rats weighing about 190 g were purchased from the pasteur institute of iran. in ...

In Refsum disease, disorders of peroxisome biogenesis, and rhizomelic chondrodysplasia punctata, pathological accumulation of phytanic acid results from impaired alpha-oxidation of this branched-chain fatty acid. Previous studies from this laboratory indicated that activation of phytanic acid to its CoA derivative precedes its alpha-oxidation in peroxisomes. It was reported that this reaction i...

BACKGROUND Liver enzymes elevations (LEE) can be observed after kidney transplantation due to multifactorial causes. OBJECTIVES We performed a retrospective study on 1589 kidney transplants, 971 male and 618 female, who were hepatitis B surface antigen (HBsAg) and hepatitis C virus-antibody (HCV Ab) negative, and had no other liver diseases, to detect the prevalence of LEE and its risk factor...

Recently it has been observed that liver enzyme activity may be markedly affected by dietary conditions. Seifter et al. (1) have demonstrated that during complete dietary protein deprivation loss of arginase and n-amino acid oxidase from rat liver occurs more rapidly than loss of general liver protein. We (2) have observed that the level of xanthine oxidase activity in rat liver may be altered ...

The clinical value and sensitivity of serum caffeine clearance measurement has been evaluated as an indicator of hepatic disease. After a 17 hour caffeine exclusion period, 300 mg of caffeine citrate was administered orally to the study subjects. Serum samples were taken four and 16 hours later. Serum caffeine concentrations were measured using an enzyme multiplied immunoassay technique (EMIT) ...