Mesenchymal stromal cells (MSCs) are non-specialized adult stem (ASCs), that reproduce to provide specific cytotypes [...]

Pluripotent hematopoietic progenitor cells (CFU-GEMM), myeloid progenitor cells (CFU-GM), and erythroid progenitors (BFU-E) were studied in midtrimester human fetuses using the mixed colony assay. All three progenitor cell populations were detected at high levels in the fetal liver from 12 to 23 wk of gestation. Stem cells were first observed in the bone marrow at 15-16 wk of gestation, althoug...

severe hepatic failure accounts for many deaths and raises medical costs each year worldwide. currently, liver transplantation is the most common therapeutic option for patients with end-stage chronic liver disease. due to decrease in the number of organ donors, many in need of transplantation continue to remain on the waiting list. hepatic tissue engineering is a step toward alleviating the ne...

objective(s): stem cell therapy is believed to be as a promising treatment strategy for tissue repair and regeneration. the plasticity specification of the adult stem cells, such as mscs, has enabled that these cells to be used in the treatment of a broad spectrum of diseases like liver disorders. in this study, the production of urea and albumin (alb), glycogen storage, and expression of some ...

Long-term cultures of murine fetal liver have been successfully established using a modification of our in vitro bone marrow culture system (14, 15). Fetal liver cells from midgestation BALB/c embryos were plated onto BAB-14 bone marrow stromal cell-adherent layers. After a 3-5 wk period, cell growth began to increase and these cells were expanded in number on fresh feeder layers. The cultured ...

objective: the neural crest is a transient structure of early vertebrate embryos that generates neural crest cells (nccs). these cells can migrate throughout the body and produce a diverse array of mature tissue types. due to the ethical and technical problems surrounding the isolation of these early human embryo cells, researchers have focused on in vitro studies to produce nccs and increase t...

cancer cell-stromal cell interaction plays a crucial role in the growth and invasion of tumor cells of various organs. it has been recently suggested that adipocytes and preadipocytes, two specific types of stromal cells, affect the biological behavior of variety of breast cancer cell lines. in spite of few investigations, there is a controversy in the literatures about the effects of adipocyte...

Background: Self-renewal, active proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, make mesenchymal stem cells to be potentially therapeutic for liver cirrhosis. Aim and Objectives: To evaluate the therapeutic potential of the combination of human Bone Marrow derived Mesenchymal Stromal Cells (BM-MSC) and silymarin in Carbon tetrachloride (CCl ) induce...

Hematopoietic stem cells (HSCs) undergo dramatic expansion during fetal liver development, but attempts to expand their numbers ex vivo have failed. We hypothesized that unidentified fetal liver cells produce growth factors that support HSC proliferation. Here we describe a novel population of CD3+ and Ter119- day-15 fetal liver cells that support HSC expansion in culture, as determined by limi...