Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...

Loop-mediated isothermal amplification (LAMP) is a gene amplification method which amplifies DNA with high specificity and efficiency under isothermal conditions. Because of its rapidity and simplicity, it is a valuable diagnostic tool in the early detection and identification of infectious diseases. LAMP method is based on the use of a set of four to six specially designed primers spanning six...

Detection of infectious viruses in mosquitoes is one the prerequisite measures to monitor prevalence vector-borne viral diseases. Determining which are currently infected with arboviruses such as Zika, dengue, and chikungunya virus not yet practical endemic areas due multiple causes including difficulty dealing virus’ unstable RNA. In this study, instead handling RNA, virus-derived DNA (vDNA) w...