objective(s): gardenia jasminoides ellis (gj, cape jasmine fruit, zhi zi) has been traditionally used for the treatment of infectious hepatitis, aphthous ulcer, and trauma; however, the direct evidence is lacking. materials and methods:we investigated the effect of the gj extract(gj) and gallic acid (ga) on lipopolysaccharide (lps) induced inflammation of bv-2 microglial cells and acute liver i...

septicaemia caused by gram-negative pathogens is a dangerous infection which is associated with high incidence of liver dysfunction. the severe and acute hepatotoxicity is presumably due to massive release of endotoxin into systemic circulation after bacterial killing. the direct toxic effect of endotoxin is probably due to the increased production of reactive oxygen intermediates as o 2 - , pe...

The objective of the present study was to investigate the clinical and histopathological effects of intravitreal injection of pentoxifylline (PTX) the management of an experimental model of uveitis. Fifty-two rabbits were divided randomly into six intravitreal treated groups as below: 1) Balanced salt solution (BSS), 2) Salmonella typhimurium lipopolysaccharide endotoxin (LPS) + BSS, 3...

Abstract We conducted a genome-wide siRNA screen for regulators of LPS-driven TNF expression in macrophages. used splicing-independent reporter the screen, and while we identified very few core spliceosome components among strongest hits, observed clear enrichment peripheral splicing regulators. Among these, novel putative regulator, UBL5, gave strong phenotype, UBL5 shRNA-expressing cell line ...

Purpose: To investigate the effect of miR-493-5p in lipopolysaccharide (LPS) -induced ATDC5 chondrogenic cells.
 Methods: The MTT assay was used to determine viability LPS-induced cells. ENCORI (starbase.sysu.edu.cn/) predict target miR-493-5p. Quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) expression controlled cells and treated with various combinations LPS, negat...

Abstract Background Acute liver failure (ALF) is associated with a high mortality rate, and there are still no effective treatments except transplantation artificial therapies. This study aimed to determine the effects, therapeutic window mechanisms of selonsertib, selective inhibitor ASK1, for ALF therapy. Results Lipopolysaccharide d -galactosamine (LPS/GalN) were used simulate ALF. We found ...

Lipopolysaccharide (LPS) is essential for most gram-negative bacteria and plays an important role in serum resistance, pathogenesis, drug protection from harsh environments. The outer core oligosaccharide of LPS involved bacterial recognition invasion host cells. D-galactosyltransferase WaaB responsible the addition D-galactose to LPS, which Salmonella typhimurium invasion. Here we report first...