Enzyme replacement therapy of lysosomal storage disorders is complicated by the lack of enzyme transport across the bloodbrain barrier (BBB). The present studies evaluate the delivery of a model enzyme across the BBB following enzyme conjugation to a BBB receptor-specific monoclonal antibody (mAb). Bacterial -galactosidase (116 kDa) was conjugated to the rat 8D3 mAb to the rat transferrin recep...

Boswellia resin is a major anti-inflammatory agent in herbal medical tradition, as well as a common food supplement. Its anti-inflammatory activity has been attributed to boswellic acid and its derivatives. Here, we re-examined the antiinflammatory effect of the resin, using inhibitor of nuclear factorB (I B ) degradation in tumor necrosis factor (TNF) -stimulated HeLa cells for a bioassay-guid...

Stomach ulceration is a major side effect of most chemopreventive drugs. We have established that although resveratrol is a promising chemopreventive compound, it delays the ulcer healing process. However, its analog hydroxystilbene-1 (HST-1) was devoid of such an ulcerogenic side effect. Consequently, here we tried to explore the chemopreventive efficacy of HST-1 compared with resveratrol in d...

16 Replication competent poxvirus vectors with an attenuation phenotype and with high 17 immunogenic capacity of the foreign expressed antigen are being pursuit as novel 18 vaccine vectors against different pathogens. In this investigation we have examined the 19 replication and immunogenic characteristics of two vaccinia virus (VACV) mutants 20 M65 and M101. These mutants were generated after ...

Bafilomycin A1, a macrolide antibiotic isolated from Streptomyces species, has been used as an inhibitor of vacuolar H ATPase (V-ATPase). Bafilomycin has been also evaluated as a potential anticancer agent because it inhibits cell proliferation and tumor growth. Although these anticancer effects of bafilomycin are considered to be attributable to the intracellular acidosis by V-ATPase inhibitio...

Tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor superfamily, which has been shown to preferentially induce apoptosis in cancer cells without adverse effects on normal cells. However, there are still some cancer cells, especially those with high malignancy, resistant to TRAIL-induced apoptosis, impeding the clinical anticancer efficiency o...

Non–small cell lung cancers (NSCLC) are associated with very dismal prognoses, and adjuvant chemotherapy, including irinotecan, taxanes, platin, and Vinca alkaloid derivatives, offers patients only slight clinical benefits. Part of the chemoresistance of NSCLCs results from the constitutive or anticancer drug-induced activation of the nuclear factor-KB (NF-KB) signaling pathways. The present st...

The antiapoptotic protein Bcl-2 is overexpressed in amajority of breast cancers, and is associated with adiminished apoptotic response and resistance to variousantitumor agents. Bcl-2 inhibition is currently beingexplored as a possible strategy for sensitizing breastcancer cells to standard chemotherapeutic agents. Anti-sense Bcl-2 oligonucleotides represent one method f...

Although stop codon readthrough is used extensively by viruses to expand their gene expression, verified instances of mammalian readthrough have only recently been uncovered by systems biology and comparative genomics approaches. Previously our analysis of conserved protein coding signatures that extend beyond annotated stop codons predicted stop codon readthrough of several mammalian genes, al...

21 In our study, we report the identification, characterization, and gene cloning of a novel 22 protein elicitor (PeBL1) secreted from Brevibacillus laterosporus strain A60. Through a 23 purification process consisting of ion exchange chromatography and HPLC, we isolated a 24 protein that was identified by ESI-QTOF-MS/MS. The 351-bp PeBL1 gene produces a 25 12833 Da protein with 116 amino acids...