As an important signal molecule, salicylic acid (SA) improves plant tolerance to aluminum (Al) stress. The objective of this study was to investigate the effects of exogenous SA application on the dynamics of endogenous SA and reactive oxygen species in soybean (Glycine max L.) exposed to Al stress. The roots of soybean seedlings were exposed to a combination of AlCl3 (30 μM) and SA (10 μM)/PAC...

The characteristics of anthocyanin and lignin are important parameters in evaluating the quality red Toona sinensis buds. Red T. buds prone to senescence during postharvest storage, which subsequently affects their sales. However, mechanism under low-temperature conditions remains unclear. In this study, were stored at 4 °C, contents as well enzyme activities PAL (phenylalanine ammonia lyase), ...

The Clostridium stercorarium F-9 pel9A gene encodes a pectate lyase Pel9A consisting of 1,240 amino acids with a molecular weight of 135,171. The mature form of Pel9A is a modular enzyme composed of two family-9 catalytic modules of polysaccharide lyases, CM9-1 and CM9-2, in order from the N terminus. Pel9A showed an overall sequence similarity to the hypothetical pectate lyase PelX of Bacillus...

Oxidative browning is a common and often severe problem in plant tissue culture systems caused by the accumulation and oxidation of phenolic compounds. The current study was conducted to investigate a novel preventative approach to address this problem by inhibiting the activity of the phenylalanine ammonia lyase enzyme (PAL), thereby reducing the biosynthesis of phenolic compounds. This was ac...

A split plot factorial 2 × 3 experiment was designed to examine and characterize the relationships among secondary metabolites (total phenolics, TP; total flavonoids, TF), carbohydrate content, C/N ratio, protein synthesis and L–phenylalanine ammonia lyase (PAL; EC 4.3.1.5) activity in the Malaysian medicinal herb Labisia pumila (Blume) FernVill. under different CO2 concentrations (400 = ambien...

the effect of salicylic acid (sa) on induction of resistance against root-knot nematode (meloidogyne javanica) and the effect of m. javanica to induce biochemical defense responses in tomato (solanum lycopersicum l.) roots at six-leaf stage were investigated. meanwhile, the effect of different concentrations of sa on mortality of second stage juveniles of m. javanica was examined. changes in th...

The accumulation of the isoflavonoid phytoalexin, glyceollin, occurs in hypocotyls of green soybean seedlings (Glycine max L. Merr. cv Harosoy 63) in response to the injection of a glucan elicitor isolated from the mycelial walls of the fungus, Phytophthora megasperma f. sp. glycinea. This accumulation, which levels off after 24 hours, is preceded by a dramatic, transient rise in extractable ac...

Chronic metabolic acidosis increases proximal tubular citrate uptake and metabolism. The present study addressed the effect of chronic metabolic acidosis on a cytosolic enzyme of citrate metabolism, ATP citrate lyase. Chronic metabolic acidosis caused hypocitraturia in rats and increased renal cortical ATP citrate lyase activity by 67% after 7 d. Renal cortical ATP citrate lyase protein abundan...

Chronic metabolic acidosis increases proximal tubular citrate uptake and metabolism. The present study addressed the effect of chronic metabolic acidosis on a cytosolic enzyme of citrate metabolism, ATP citrate lyase. Chronic metabolic acidosis caused hypocitraturia in rats and increased renal cortical ATP citrate lyase activity by 67% after 7 d. Renal cortical ATP citrate lyase protein abundan...

Using in situ R N A hybridization and immunohistochemical techniques, we investigated the tissue-specific and light-dependent expression o f four genes involved in phenylpropanoid metabolism in various parts o f parsley ( Petroselinum crispum) seedlings. The genes encode phenylalanine ammonia-lyase (PAL) and 4-coumarate: Co A ligase (4 CL), two enzymes o f general phenylpropanoid metabolism; ch...