I . Adult rats were fed on diets free of either lysine, methionine, threonine or protein. The threonineand protein-deficient animals lost weight at approximately the same rate, about IOO g in 14 weeks, at which time several were moribund. In contrast, lysine-deficient animals lost only about 3 0 g in 14 weeks and had lost only46 g after zz weeks, when they were killed. Methioninedeficient anima...

A mutant of Salmonella typhimurium was selected for its spontaneous resistance to the lysine analog, thialysine (S-2-aminoethyl cysteine). This strain, JB585, exhibits a number of pleiotropic properties including a partial growth requirement for threonine, resistance to thiaisoleucine and azaleucine, excretion of lysine and valine, and inhibition of growth by methionine. Genetic studies show th...

Posttranslational modifications (PTMs) of tubulin specify microtubules for specialized cellular functions and comprise what is termed a "tubulin code." PTMs of histones comprise an analogous "histone code," although the "readers, writers, and erasers" of the cytoskeleton and epigenome have heretofore been distinct. We show that methylation is a PTM of dynamic microtubules and that the histone m...

In order to describe the response of broiler breeder hens to dietary amino acids and to develop an effective model for the precise feeding of these birds after sexual maturity, accurate estimates of the amounts of each amino acid required for maintenance and egg production are needed. The maintenance requirement for threonine and lysine were estimated in two different experiments by measuring t...

Pipecolic acid is a component of several secondary metabolites in plants and fungi. This compound is useful as a precursor of nonribosomal peptides with novel pharmacological activities. In Penicillium chrysogenum pipecolic acid is converted into lysine and complements the lysine requirement of three different lysine auxotrophs with mutations in the lys1, lys2, or lys3 genes allowing a slow gro...

One hundred fourteen high-lean growth gilts (72.5 kg BW) were used to determine the apparent digestible lysine requirement for maximum growth performance and carcass protein deposition rate from 72.5 to 136 kg BW. The experiment was a randomized complete block design with initial BW used to establish blocks. Six dietary treatments were included, ranging from .44 to .94% (.10% increments) appare...

effect of dietary free l-lysine on growth, food intake, and muscle composition of beluga juveniles were investigated over 6 weeks. control diet lysine content was 2.1% of dry matter (4.4% of dietary protein). three experimental diets were prepared by adding lysine (0.5, 1 and 1.5%) to control diet to obtain diets containing 2.6, 3.1 and 3.6% of dry matter lysine (corresponding to 5.5, 6.6 and 7...

We conducted three experiments to determine the sulfur amino acid (SAA) and methionine requirements of finishing gilts. Gilts (PIC Line 326 x C-15, Exp. 1; Line 326 x C-22, Exp. 2 and 3) were blocked by initial weight in randomized complete block designs. In Exp. 1, 64 gilts (initially 54 kg) were fed diets containing either .56 or .44% apparent digestible lysine with increasing SAA levels (63,...

5-Aminolaevulinic acid dehydratase catalyses the formation of porphobilinogen from two molecules of 5-aminolaevulinic acid. The studies described highlight the importance of a bivalent metal ion and two active-site lysine residues for the functioning of 5-aminolaevulinic acid dehydratase. Dehydratases fall into two main categories: zinc-dependent enzymes and magnesium-dependent enzymes. Mutatio...