L-malate, a tricarboxylic acid cycle (TCA) intermediate, plays an important role in transporting NADH from cytosol to mitochondria for energy production and may be involved in the beneficial effects of improving physical stamina. In the present study, we investigated the effects of L-malate on the performance of forced swimming time and blood biochemical parameters related to fatigue - blood ur...

the objectives of this study were to determinethe interactions between two hepatic enzymes and some minerals in the liver of broiler chickens. the study was performed with male and female from 1 to 56 days of age of broiler chickens. malic acid was added to the water and offered to chickens freely from the start to the end of the experiment with constant concentration. the treatments consisted ...

Schizosaccharomyces pombe is of interest to wine-makers because, unlike the Saccharomyces cerevisiae wine yeasts, it eciently metabolizes all levels of malic acid found in grape musts. To determine the metabolic pathway for malatemetabolism in Sch. pombe, a mutational analysis was performed using a selection system which isolated 154mutants that were unable to utilize malic acid. Themutants we...

The structure of apo malate dehydrogenase from Escherichia coli has been determined to 1.45 A resolution. The crystals belonged to space group C2, with unit-cell parameters a = 146.0, b = 52.0, c = 168.9 A, beta = 102.2 degrees. The structure was determined with the molecular-replacement pipeline program BALBES and was refined to a final R factor of 18.6% (R(free) = 21.4%). The final model has ...

Changes in levels of isocitrate lyase, malate synthase, and catalase have been investigated during germination of flax (Linum usitatissimum L.) in the presence and absence of itaconate. Germination was accompanied by a rapid increase in these enzymes during the first 3 days. The presence of 38 millimolar itaconate inhibited the incidence of seed germination and the growth of embryo axes as well...

Effects of ADP and ATP on products of malate oxidation in the presence or absence of respiratory inhibitors and an uncoupler were investigated in mitochondria isolated from mung bean (Phaseolus aureus var. Jumbo) hypocotyls. Changes in levels of products from malate oxidation generally correlated directly with changes in oxygen uptake. Effects of ADP and ATP were indistinguishable from each oth...

The activity of several photosynthetic enzymes was unaltered by exposure of sorghum or maize to low temperatures (10 C) and light (170 w m(-2)). Two light-activated C(4)-pathway enzymes, NADP-malate dehydrogenase and pyruvate Pi dikinase, were reduced in activity, and this was largely attributable to a loss of enzyme rather than to incomplete enzyme activation. Loss of NADP-malate dehydrogenase...

The activities of mitochondrial malate dehydrogenase (mMDH) and the total mitochondrial proteins increase as a function of body mass in the freshwater catfish, Clarias batrachus. It clearly indicates an increase in energy production in larger-sized individuals for various purposes including prey-predator interactions. The higher activity of lactate dehydrogenase (LDH) in larger fish may indicat...

An immobilized three-enzyme system, malate dehydrogenase (EC 1.1.1.37)-citrate synthase (EC 4.1.3.7)-lactate dehydrogenase (EC 1.1.1.27), was investigated as a model for the rate of oxalacetate production and utilization in mitochondria. Lactate dehydrogenase is included to mimic the NADH-utilizing system of mitochondria. This three-enzyme system was immobilized in three different ways (1) on S...

Pyruvate orthophosphate dikinase, phosphoenolpyruvate carboxylase, and NADP-malate dehydrogenase function in a series of reactions for fixing Con in mesophyll cells and NADP-malic enzyme (ME) catalyzes the production of CO, and NADPH in bundle sheath cells of maize which is a NADP-ME type C4 plant. Northern blot analyses with cDNA clones for pyruvate orthophosphate dikinase and phosphoenolpyr...