Human pluripotent stem cell (hPSCs) derived-pancreatic islets (hSC-islets) are good candidates for replacement therapy patients with diabetes as substitutes deceased donor-derived islets, because they and have infinite proliferation potential. Grafted hSC-islets ameliorate hyperglycemia in diabetic mice; however, several weeks needed to normalize the hyperglycemia. These data suggest require ma...

Background: Setting up an in vitro follicle culture system that resembles in vivo ovary condition has high value in research. Additionally, expression evaluation of folliculogenesis involved genes could lead us to the designing of better culture system. Materials and Methods: ovaries of 12-day-old female NMRI mice were removed, 100-130 μm pre-antral follicles were mechanically isolated from fre...

Background: Polycystic ovary syndrome (PCOS) is a common complex genetic endocrinopathy, affecting 5 - 10% of women at reproductive age. PCO granulosa cells seem to have abnormal responses to follicle- stimulating hormone (FSH). FSH is considered to be a pituitary glycoprotein that plays an important role during folliculogenesis as it promotes the proliferation and differentiation of granulosa ...

The differentiation of mesenchymal cells into chondrocytes and chondrocyte proliferation and maturation are fundamental steps in skeletal development. Runx2 is essential for osteoblast differentiation and is involved in chondrocyte maturation. Although chondrocyte maturation is delayed in Runx2-deficient (Runx2(-/-)) mice, terminal differentiation of chondrocytes does occur, indicating that add...

Primary cultures of fetal rat calvarial osteoblasts undergo a developmental sequence with respect to the temporal expression of genes encoding osteoblast phenotypic markers. Based on previous suggestions that gene-nuclear matrix associations are involved in regulating cell- and tissue-specific gene expression, we investigated the protein composition of the nuclear matrix during this development...

The discovery of Aire-dependent transcriptional control of many tissue-restricted self-antigen (TRA) genes in thymic epithelial cells in the medulla (medullary thymic epithelial cells, mTECs) has raised the intriguing question of how the single Aire gene can influence the transcription of such a large number of TRA genes within mTECs. From a mechanistic viewpoint, there are two possible models ...

bovine ephemeral fever (bef) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of asia, australia, and africa. in recent years, bef has been distributed in many provinces of iran and caused economic losses. in this study the sequence encoding the g1 epitope of bovine ephemeral fever virus (befv) g glycoprotein was amplified by pcr, ligated into th...

introduction: kainic acid (ka) induces neuropathological changes in specific regions of the mouse hippocampus comparable to changes seen in patients with chronic temporal lobe epilepsy (tle). according to different studies, the expression of a number of genes are altered in the adult rat hippocampus after status epilepticus (se) induced by ka. this study aimed to quantitatively evaluate changes...

background: after radiation therapy (rt), some health hazards including dna damages may occur where melatonin can play a protective role due to free radical generation. on the other hand, serious accidental overexposures may occur during rt due to nuclear accidents which necessitate the need for study on exposure to high-dose radiations during treatments. objective: the aim of this study was to...