Ficolins are thought to be pathogen-associated-molecular-pattern-(PAMP-) recognition molecules that function to support innate immunity. Like mannose-binding lectins (MBLs), most mammalian ficolins form complexes with MBL-associated serine proteases (MASPs), leading to complement activation via the lectin pathway. However, the ability of murine ficolin B, a homologue of human M-ficolin, to perf...

The metallo-β-lactamase (MBL) GOB-1 was expressed via a T7 expression system in Escherichia coli BL21(DE3). The MBL was purified to homogeneity and shown to exhibit a broad substrate profile, hydrolyzing all the tested β-lactam compounds efficiently. The GOB enzymes are unique among MBLs due to the presence of a glutamine residue at position 116, a zinc-binding residue in all known class B1 and...

Sir, Enterobacteriaceae producing metallo-b-lactamases (MBLs), and particularly VIM-type MBLs, have frequently been implicated in hospital outbreaks across Europe, blaVIM genes having been linked to Tn402 derivatives, epidemic plasmids (IncN, IncI1, IncHI2) and occasionally with particular Enterobacteriaceae clones. VIM enzymes have been classified in three clusters (VIM-1, VIM-2 and VIM-7) acc...

Carbapenems, mainly imipenem and meropenem, are potent agents for the treatment of infections due to multiresistant Pseudomonas sp. clinical isolates. However, the prevalence of carbapenem resistance in this genus has been increasing worldwide. High-level resistance to carbapenems ( 32 g/ml) is still uncommon in Pseudomonas spp. but can be due to the presence of Ambler class B -lactamases—metal...

conclusions the inter- and intra-hospital dissemination of resistant clones is a matter of concern and is an indicator of the level of the improvement and surveillance of standard hygiene, particularly disinfection and hand washing before and after contact with patients. given the emergence of mbl-producing strains, surveillance has become an important procedure to control the transmission of r...

Results from propagation measurements, conducted in an indoor office environment at 2.4, 4.75, and 11.5 GHz, are presented. The data were obtained in small clusters of six measurements, using a coherent wideband measurement system. The channel characteristics for the three frequencies are compared by evaluating path loss, rms delay spread, and coherence bandwidth. An analytical model for evalua...

background one of the major clinical problems regarding pseudomonas aeruginosa is attributed to metallo-beta-lactamases (mbl). this group of enzymes is a subset of beta lactamases which belong to group b of ambler classification and cause hydrolysis of carbapenems. based on epidemiological studies conducted worldwide, it is proved that prevalence of genes coding mbls in p. aeruginosa species ar...

aim : the aims of this study were to determine the phylogenetic groups and investigate phenotypic and genotypic of esbl and mbl-producing e . coli isolates from patients with diar­rhea in kerman, southeast of iran. background : escherichia coli is detected as a significant cause of sporadic and outbreaks of diarrhea. the emergence of extended-spectrum ?-lactamases (esbls) and metallo-?-lactamas...