High resolution melt (HRM) is gaining considerable popularity as a simple and robust method for genotyping sequence variants. However, accurate genotyping of an unknown sample for which a large number of possible variants may exist will require an automated HRM curve identification method capable of comparing unknowns against a large cohort of known sequence variants. Herein, we describe a new ...

This study describes a new approach in the screening for loss-of-gene mutants in Heavy Ion Bombardment (HIB) mutant populations of genetically complex organisms such as hexaploid bread wheat using multiplexed single-color (SYBR Green) melt curve analyses. The assay was set up for three target genes to test its validity and applicability. For each gene, three genome-specific primer pairs (one fo...

Mutations in BCR-ABL kinase domain are associated with resistance to tyrosine kinase inhibitors in some patients with chronic myeloid leukemia. Therefore, mutation detection becomes essential in such patients. We aimed to apply high-resolution melt curve analysis (HRM) for a rapid screening prior to sequencing to select only mutation positive samples. One hundred and one samples with different ...

[This corrects the article DOI: 10.1371/journal.pone.0190192.].

Volumetrically significant melt production requires crustal temperatures above approximately 800 °C. At the grain scale, the former presence of melt may be inferred based on various microstructures, particularly pseudomorphs of melt pores and grain-boundary melt films. In residual migmatites and granulites, evidence of melt-extraction pathways at outcrop scale is recorded by crystallized produc...

The chemical diffusion of fluorine in jadeite melt has been investigated from 10 to 15 kbars and 1200 to 14OO“C using diffusion couples of jadeite melt and fluorine-bearing jadeite melt (6.3 wt.% F). The diffusion profile data indicate that the diffusion process is concentration-independent, binarv. F-O interdiffision. The F-O interdiffusion coefficient ranges from 1.3 X IO-’ to 7.1 X lo-’ cm*/...