in this study, our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented...

background: bone marrow-derived mesenchymal cellscan transdifferentiate into cardiomyocyte cells and improveheart function after transplantation. since biomaterials canimprove the cell retention in the site, cell survival and differentiation,heart tissue engineering is now being explored as anapplied solution to support cell-based therapies and increasetheir efficacy for myocardial diseases. ch...

multipotent mesenchymal stem cells (mscs) are recently found to alter the tumor condition. however their exact role in tumor development is not yet fully unraveled. mscs were established to perform many of their actions through paracrine effect. thus investigation of msc secretome interaction with tumor cells may provide important information for scientists who are attempting to apply stem cell...

BACKGROUND/AIMS Hematopoiesis can occur in the liver, when the bone marrow fails to provide an adequate environment for hematopoietic stem cells. Hepatic stellate cells possess characteristics of stem/progenitor cells, but their contribution to hematopoiesis is not known thus far. METHODS Isolated hepatic stellate cells from rats were characterized with respect to molecular markers of bone ma...

INTRODUCTION Adult mesenchymal stem cell therapy has a potential application in the biological treatment of disc degeneration. Our objectives were: to direct adipose-derived mesenchymal stem cells (AD-MSC) from the sand rat to produce a proteoglycan and collagen type I extracellular matrix (ECM) rich in known ECM components of the annulus fibrosis of disc; and to stimulate proteoglycan producti...

Based on a variety of preclinical studies showing that mesenchymal stem cells (MSC) play a significant role in tissue repair and homeostasis, MSC have rapidly moved into a phase of clinical trials investigating their efficacy as a cell-based therapeutic modality for a diverse group of applications. An emerging body of evidence shows that in addition to being a progenitor cell population with se...

Direct cellular interactions of MDA-MB-231cherry breast cancer cells with GFP-transduced human mesenchymal stroma/stem-like (MSCGFP) in a co-culture model resulted spontaneous cell fusion by the generation MDA-MSC-hyb5cherry GFP hybrid cells. The proliferative capacity MDA-MSC-hyb5 was enhanced about 1.8-fold when compared to parental In contrast induced tumor development vivo within 18.8 days,...

Lung diseases are a major cause of global morbidity and mortality that are treated with limited efficacy. Recently stem cell therapies have been shown to effectively treat animal models of lung disease. However, there are limitations to the translation of these cell therapies to clinical disease. Studies have shown that delayed treatment of animal models does not improve outcomes and that the m...

Submit Manuscript | http://medcraveonline.com Abbreviations: iPSC: Induced Pluripotent Stem Cells; ESC: Embryonic Stem Cells; SCNT: Somatic Cell Nuclear Transfer; TFs: Transcription Factors; Sox2: Sex Determining Region Y-box 2; Klf4: Krueppel-like factor 4; Utf1: Undifferentiated Embryonic Cell Transcription factor 1; Sall4: Sal-like protein 4; hTERT: Telomerase Reverse Transcriptase; HAT: His...

Continued improvements in the understanding and application of mesenchymal stem cells (MSC) have revolutionized tissue engineering. This is particularly true within the field of skeletal regenerative medicine. However, much remains unknown regarding the native origins of MSC, the relative advantages of different MSC populations for bone regeneration, and even the biologic safety of such unpurif...