Several ninhydrin-positive lipids were found in methanogenic bacteria and the structure of one of them, designated as PNL2 from Methanobrevibacter arboriphilus, was identified as a diphytanyl ether analog of phosphatidylserine. The chromatographic behavior of the lipid on thin-layer plates and on a DEAE-cellulose column was identical to the ester form of phosphatidylserine. The infrared spectra...

Dairy cows experience dramatic changes in host physiology from gestation to lactation period and dietary switch from high-forage prepartum diet to high-concentrate postpartum diet over the transition period (parturition +/- three weeks). Understanding the community structure and activity of the rumen microbiota and its associative patterns over the transition period may provide insight for e.g....

Members of the domain Archaea contribute about 0.3 to 3.3% of the microbial small subunit (16S and 18S) rRNA in the rumen (22, 39, 60). Archaea have a range of different metabolisms and are found in many habitats (6), but those known to exist in the rumen are strictly anaerobic methanogens. Yanagita et al. (59) observed that 2.8 to 4.0% of ruminal microorganisms displayed autofluorescence chara...

Pet ownership positively influences clinical outcomes in cardiovascular prevention. Additionally, disease (CVD) has been previously linked to microbiota dysbiosis. We evaluated the influence of owning a pet and its relationship with intestinal microbiota. analyzed gut from 162 coronary patients CORDIOPREV study (NCT00924937) according whether they owned pets (n = 83) or not 79). The pet-owner g...

ABSTRACT The gut microbiota composition is influenced by the diet as well environment in both wild and domestic animals. We studied effects of two feeding systems on rumen hindgut microbiome semi-feral Tibetan goats kept at high altitude (∼4800 m) using 16S rRNA gene metagenomic sequencing. Intensive drylot resulted significantly higher zootechnical performance, narrower ruminal acetate: propio...

Background & Aim Sarcopenia is frequent in cirrhosis and associated with unfavourable outcomes. The role of the gut-liver-muscle axis this setting has been poorly investigated. aim study was to identify gut microbiota, metabolic inflammatory signatures sarcopenia cirrhotic patients. Methods Fifty patients assessed for presence by quantification muscle mass strength were compared age- sex-matche...

We evaluated the effects of propylene glycol (PG) on in vitro ruminal fermentation, methanogenesis, and microbial community structure. A completely randomized design was conducted incubation, 4 culture PG dose levels (0, 7.5, 15, 22.5 μL/g dry matter) were used trial. Based fermentation results, control group (0 matter, CON) second treatment (15.0 TRT) chosen for further analysis to explore bac...