The Asn-linked oligosaccharides of the pituitary hormone lutropin (LH) contain both sulfate and GalNAc. Bovine pituitary explants incorporate [3H]glucosamine, [3H]mannose, [3H]fucose, and [35S]sulfate into the Asn-linked oligosaccharides of LH. Endoglycosidase F or N-glycanase releases the [3H]glucosamine- and [3H]mannose-labeled oligosaccharides from the protein, which resolve on anion-exchang...

(tetrahydrofuran) at 703C for 16 h. The ester residue is then converted to a 6,6-dideuteriohexose residue, the O-methyl ethers of which are easily distinguishable by GC-MS of the derived alditol acetates. An alternative to acid hydrolysis that is applicable to most polysaccharides and glycoconjugates, including those containing acid-labile residues or glycosidic linkages resistant to hydrolysis...

Methanolysis of unsaturated fatty acids and triglycerides was carried out with boron trihalide-methanol reagents of various ages. Boron trichloride-methanol produced esters apparently free from contaminants; boron trifluoride caused some loss of highly unsaturated esters after 90 min at 100 or 120 degrees C in Teflon-lined screw-cap vials and at 120 degrees C in ampoules. The losses were more m...