In developing countries, people mainly depend on rice as their primary source of calories. However, the thiamine content is below minimal requirements. Biofortification, via genetic engineering, a cost-effective strategy to increase in rice. We report optimization matrix-specific method, including extensive sample preparation ensure maximal sensitivity and stability. The LC-MS/MS method was ful...

This study aims to investigate the effect and mechanism of action of cinnamic acid (CA) on the proliferation and apoptosis of leukaemia K562 cells. Morphological changes in cells were observed in K562 cells treated by CA with different doses (1.5, 3.0, and 6.0 mg/mL). Cell proliferation capabilities were measured by methyl thiazolyl tetrazolium assay. Cell cycle was determined by flow cytometry...

Oral cancer threats people's life and health seriously. Traditional treatment (surgery, radiotherapy, chemotherapy and traditional Chinese medicine treatment) is lack of pertinence that affects curative effect and prognosis. Therefore, it is necessary to explore the specific targets for oral cancer treatment. MiRNA-139 was transfected into oral cancer Tca8113 cells. Methyl thiazolyl tetrazolium...

Triazinopyrazolothieno pyrimidine derivatives 1-5 were evaluated for their anti-inflammatory, analgesic and anticancer activities and acute toxicity. Anti-inflammatory activity of the compounds was studied using the carrageenan test. All tested compounds showed analgesic activity, 3-methoxycarbonyl-4,6-dimethyl-8-[(N-methylindolyl)methyl] pyrimido [5',4':4,5]thieno [3',2'-3,4]pyrazolo [5,1-c]tr...

It has now been firmly established by several groups of workers (1-10) that in yeast thiamine is enzymatically synthesized from pyrimidine pyrophosphate and thiazole monophosphate. However, there is little information about the biosynthetic pathways of pyrimidine and thiazole themselves. Thiamine was found as the first natural com pound containing a thiazole ring. Recently, the presence of the ...