This paper describes seasonal changes of microcystin-LR (MC-LR) and its glutathione (MC-LR-GSH) and cysteine conjugates (MC-LR-Cys) in three aquatic animals--snail (Bellamya aeruginosa), shrimp (Macrobrachium nipponensis) and silver carp (Hypophthalmichthys molitrix) collected from Lake Taihu, China. MC-LR, MC-LR-GSH, and MC-LR-Cys were determined by liquid chromatography electrospray ionizatio...

Biolistic gun-mediated transformation is one of the two most effective and popular methods for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature e...

BACKGROUND Lung mast cells are stereotypically divided into connective tissue (MC(TC)) and mucosal (MC(T)) mast cells. This study tests the hypothesis that each of these subtypes can be divided further into site-specific populations created by the microenvironment within each anatomical lung compartment. METHODS Surgical resections and bronchial and transbronchial biopsies from non-smoking in...

The study was carried out to establish an EV71 virus culture system on Vero cells in a shake (Wave bioreactor) incorporating microcarriers for the production of hand, foot and mouth (HFM) vaccine caused by virus. results investigation 2 types (Cytodex 1 Cytodex 3), showed that gave better efficiency when culturing suspension conditions. combination with Wave bioreactor cell over 3x106cells/ml. ...

Background: Monte Carlo (MC) modeling of a linear accelerator is a prerequisite for Monte Carlo dose calculations in external beam radiotherapy. In this study, a simple and efficient model was developed for Elekta SL-25 linear accelerator using MCNP4C Monte Carlo code Materials and methods: The head of Elekta SL-25 linac was simulated for 6 and 18 MV photon beams using MCNP4C MC code. Energ...

This paper introduces the principles of interferometry to multicarrier code division multiple access (MC-CDMA). Specifically, we propose the use of MC-CDMA with novel carrier interferometry (CI) complex spreading codes. The CI/MC-CDMA method, applied to mobile wireless communication systems, offers enhanced performance and flexibility relative to MC-CDMA with conventional spreading codes. Speci...