Though cytoplasmic male sterility (CMS) in peppers is associated with the orf507 gene, definitive and direct evidence that it directly causes male sterility is still lacking. In this study, differences in histochemical localization of anther cytochrome c oxidase between the pepper CMS line and maintainer line were observed mainly in the tapetal cells and tapetal membrane. Inducible and specific...

The aim of the present work was the characterization of nuclear bodies in the microspore and developing pollen cells of Hyacinthus orientalis L.. The combination of Ag-NOR, immunofluorescence and immunogold techniques was used in this study. The obtained results showed the presence of highly agyrophylic extranucleolar bodies in microspore and developing pollen cells, which were finally identifi...

The fungicide phosphite was found to reduce pollen fertility in Australian and exotic species. In the perennial Dryandra sessilis the reduction was evident for a year after treatment with 2.5-10 gL,1 phosphite sprayed to run off. Pterochaeta paniculata, an annual, showed reduced pollen fertility in flowers that opened 16-30 days after spraying. The horticultural species Pelunia hybrida and Trad...

Heat stress can induce the cultured microspores into embryogenesis. In this study, whole genome bisulphite sequencing was employed to study global DNA methylation variations after short-term heat shock (STHS) treatments in cultured microspores of Brassica napus cv. Topas. Our results indicated that treatment on cultured Topas microspores at 32 °C for 6 h triggered DNA hypomethylation, particula...

The phytohormone gibberellin (GA) is critical for anther development. RGA, a member of the DELLA family proteins that are central GA signalling repressors, key regulator male fertility in plants. However, downstream genes GA–RGA-mediated development remain to be characterised. We identified RGA Target 1 (RGAT1), novel Arabidopsis gene, functions as an important RGA-regulated target pollen RGAT1...

Under specific stress treatments (cold, starvation), in vitro microspores can be induced to deviate from their gametophytic development and switch to embryogenesis, forming haploid embryos and homozygous breeding lines in a short period of time. The inductive stress produces reactive oxygen species (ROS) and nitric oxide (NO), signalling molecules mediating cellular responses, and cell death, m...

BACKGROUND AND AIMS Global warming is gaining significance as a threat to natural and managed ecosystems since temperature is one of the major environmental factors affecting plant productivity. Hence, the effects of moderate temperature increase on the growth and development of the tomato plant (Lycopersicon esculentum) were investigated. METHODS Plants were grown at 32/26 degrees C as a mod...

Microspores can be induced to develop homozygous doubled haploid plants in a single generation. In the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. Two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with Agrobacterium tumefaciens. Di...

microspore culture was applied to induce embryogenesis in roses (rosa hybrida) cv. hav1. important factors affecting viability of isolated microspores and formation of multi-cellular structures including isolation media (nln, ab, b) and induction media were studied. the most responsive developmental stage of microspores (late unicellular) was determined using dapi staining. the least infection ...