Acrylic acid (AA) is used widely in the synthesis of esters essential in the production of paints, adhesives, plastics, and coatings. The minimal systemic toxicity of AA is attributed to its rapid oxidation to acetyl-CoA and CO2 via the vitamin B12-independent beta-oxidation pathway. This oxidation is localized to the mitochondria and preliminary evidence suggests a possible inhibition of mitoc...

This article describes the rapid and diversified synthesis of pyrrolidinyl triazoles for the discovery of mitochondrial permeability transition pore (mPTP) blockers. The 1,3-dipolar cycloaddition of ethynyl trifluoroborate with azidopyrrolidine produced a key intermediate, triazolyl trifluoroborate 4, which subsequently underwent a Suzuki-Miyaura coupling reaction to afford a series of 1,4-disu...

Submit Manuscript | http://medcraveonline.com Abbrevations: PBEF: Pre-B cell colony Enhancing Factor; NAD: Nicotinamide Adenine Dinucleotide; Sir2: Silent information regulator 2; NMN: Nicotinamide Mononucleotide; GLP-1: Glucagon Like Peptide-1; SAT: Subcutaneous Adipose Tissue; VAT: Visceral Adipose Tissue; FSIGT: Frequently Sampled Intravenous Glucose Tolerance; IMCL: Intramyocellular Lipid; ...

Hepatitis B Virus X Protein Modulates Apoptosis in Primary Rat Hepatocytes by Regulating both NFB and the Mitochondrial Permeability Transition Pore Amy J. Clippinger, Tricia L. Gearhart, and Michael J. Bouchard* Graduate Program in Molecular and Cellular Biology and Genetics and Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania ...

In this study, we focused on an analysis of biguanides effects on mitochondrial enzyme activities, mitochondrial membrane potential and membrane permeability transition pore function. We used phenformin, which is more efficient than metformin, and evaluated its effect on rat liver mitochondria and isolated hepatocytes. In contrast to previously published data, we found that phenformin, after a ...

Eriksson, Ove, Piero Pollesello, and Erika Geimonen. Regulation of total mitochondrial Ca21 in perfused liver is independent of the permeability transition pore. Am. J. Physiol. 276 (Cell Physiol. 45): C1297–C1302, 1999.— Triggering of the permeability transition pore (PTP) in isolated mitochondria causes release of matrix Ca21, ions, and metabolites, and it has been proposed that the PTP media...

Calpain 1 (CPN1) is a ubiquitous cysteine protease that exists in both cytosol and cardiac mitochondria. Mitochondrial CPN1 (mit-CPN1) is located in the intermembrane space and matrix. Activation of mit-CPN1 within the intermembrane space increases cardiac injury by releasing apoptosis-inducing factor from mitochondria during ischemia-reperfusion (IR). We asked if activation of mit-CPN1 is invo...

This study evaluated the respiratory indexes (respiratory control ratio [RCR] and ADP/O ratio), mitochondrial transmembrane potential (DeltaPsim), repolarization lag phase, repolarization level, ATP/ADP ratio, and induction of the permeability transition pore of brain mitochondria isolated from normal Wistar and GK diabetic rats of different ages (1.5, 12, and 24 months of age). The effect of a...

1. Mitochondrial mechanisms involved in veratridine-induced chromaffin cell death have been explored. 2. Exposure to veratridine (30 micro M, 1 h) produces cytochrome c release to the cytoplasm that seems to be mediated by superoxide anions and that is blocked by cyclosporin A (10 micro M), MnTBAP (10 nM), catalase (100 IU ml(-1)) and vitamin E (50 micro M). 3. Following veratridine treatment, ...