Mailing address: Luiz Alberto Benvenuti • Rua Madalena, 477/31 Vila Madalena 05434-090 São Paulo, SP Brazil E-mail: [email protected] Manuscript received May 15, 2009; revised manuscript received July 25, 2009; accepted October 07, 2009. 3 males). These samples were routinely processed and paraffin-embedded. Serial 4-μm sections were stained by hematoxylin and eosin, Masson’s trichrome or su...

Mallory's triple staining is a histochemical technique used mainly for analysing connective tissues and glands and other tissues. We have described the differences in the nuclear staining between eutopic and ectopic endometrium as well as endometrial hyperplasia and adenocarcinoma using the Mallory's method. The ultrastructural differences between eutopic and ectopic endometrium have been detec...

PURPOSE To develop a digital image processing method to quantify structural components (smooth muscle fibers and extracellular matrix) in the vessel wall stained with Masson's trichrome, and a statistical method suitable for small sample sizes to analyze the results previously obtained. METHODS The quantification method comprises two stages. The pre-processing stage improves tissue image appe...

BACKGROUND Postoperative adhesion is one of the major complications of strabismus surgery and result in motility dysfunction that brings unpredictable surgical outcomes. However, there was no definitely accepted treatment method to prevent postoperative adhesion. METHODS A prospective, randomized, controlled experimental animal study was performed. Both eyes of each of 14 New Zealand White ra...

OBJECTIVES The biological functions of transforming growth factor-β signaling that involves Smad proteins have not been previously investigated with respect to coronary artery bypass grafts. The aim of the present study was to observe the immunostaining of proteins that are related to this signaling pathway. METHODS Fifteen remnants of coronary artery bypass grafts, including nine saphenous v...

PURPOSE To devise a means of providing controlled resistance between the anterior chamber and the subconjunctival space after trabeculectomy by implantation of a biodegradable, porous collagen matrix. METHODS Matrices were implanted in the right eyes of 17 rabbits after trabeculectomy, while left eyes served as surgical controls. The scleral flap was sutured loosely, and the implant provided ...

BACKGROUND Hydrosalpinx (HSP), characterized by abnormal fluid accumulation in the Fallopian tube, is one of the main causes of infertility in women; however, the mechanism underlying the formation of hydrosalpinx fluid (HF) remains elusive. The present study investigated the possible involvement of cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-dependent chloride channel, i...

BACKGROUND Research demonstrates a link between obesity and increased circulating inflammatory cytokines, which lead to changes in the microvasculature. Massive weight loss patients often experience delayed wound healing after body-contouring procedures; however, no studies exist to explore the inflammatory response of massive weight loss on microvasculature. This study hypothesized that massiv...

Intestinal parasitosis is still an important public health problem. The aim of this study was to determine the prevalence of intestinal parasitosis in children with gastrointestinal symptoms, and to evaluate its association with socio-economic and environmental factors. Stool samples of 3,216 children were examined by the saline-iodine method and trichrome staining. The cellophane tape method w...