1. ac-N-Methyl-oc-N-toluene-p-sulphonyl-L-lysine ,B-naphthyl ester (MTLNE) was synthesized as its hydrobromide and shown to be slowly hydrolysed by bovine pancreatic trypsin. The acylation step, however, is so much faster than deacylation of the acyl-enzyme that spectrophotometric measurement of the 'burst' of ,B-naphthol provides a convenient method for determining the absolute molarity of try...

We have employed dynamic light scattering and X-ray small-angle scattering for monitoring the cluster formation of hen-egg white lysozyme solutions under crystallization conditions. From time-resolved dynamic light scattering experiments we can obtain a set of observables that exhibit clear extrema, when simultaneously plotted as functions of protein and electrolyte molarity. Close to these ext...

–Flyash based geopolymer concrete is introduced in 1979 by Davidovits to reduce the use of OPC in concrete. Geopolymer is an inorganic alumino-hydroxide polymer synthesized form predominantly silicon and aluminum materials of geological origin and by product materials such as flyash (with low calcium). In this paper an attempt is made to study strength properties of geopolymer concrete using lo...

Several group A coxsackieviruses (A13, 15, 18, and 21), but not polioviruses or group B coxsackieviruses, are rapidly inactivated in low ionic strength solutions at neutral pH. The extent of inactivation is dependent upon temperature and molarity. Virions inactivated in this manner contain a normal complement of infectious RNA which remains in a state resistant to the action of ribonuclease. Ho...

A Ca2+-activated cysteine proteinase (calpain II) was purified from chicken gizzard smooth muscle by use of isoelectric precipitation, (NH4)2SO4 fractionation, chromatography on DEAE-Sepharose CL-6B, Reactive-Red 120-agarose and Mono Q. The apparent second-order rate constants for the inactivation of calpain by a series of structural analogues of L-3-carboxy-trans-2, 3-epoxypropionyl-leucylamid...

1. A method is described for fractionating alkaline and enzymic hydrolysates of ribonucleic acids and oligonucleotides on DEAE-cellulose columns by using a constant-composition eluent. 2. The relative partition coefficients of the four major and several minor nucleotides present in nucleic acid digests are given. 3. The influence of pH and of molarity of the buffer on the separation is describe...

Abstract: Chromatographic measurements were made for the adsorption of benzene, toluene and m-xylene on molecular sieving carbon (MSC) in a supercritical fluid CO2 mixed with organics. A supercritical chromatograph packed with MSC was used to detect pulse responses of the organics. Adsorption equilibria and adsorption dynamic parameters for organics were obtained by moment analysis of the respo...

Introduction: Single chain variable fragment (scFv) antibodies are reduced forms of the whole antibodies that could be regarded as an alternative tool for diagnostic and therapeutic purposes. The optimization of processes and environmental conditions is necessary to increase the production yields and enhance the productivity. This can result in a cost-effective process and respond to the high d...

The present study focused on developing Alkali Activated Fine Aggregate (AAFA) by palletization method. AAFA was manufactured using Ground Granulated Blast Furnace Slag (GGBS) and alkali activator solution at ambient conditions. Sodium hydroxide of six molarity sodium silicate to ratio 2.5 used. AACA's properties are similar Natural except for flakiness elongation index. water absorption Abrasi...