A molecule-based characterization methodology was developed for correlation and prediction of assays and properties for crude oil and petroleum fractions. From readily accessible crude oil, the assay data such as distillation curve, API gravity, and PNA contents, identifies an optimal set of chemical compositions of model hydrocarbon molecules that were designed to mimic measurable physical and...

Objectives Shepard’s mouse footpad (MFP) assay is the definitive method for ascertaining Mycobacterium leprae viability; however, this technique laborious and prolonged. Alternate methods that alleviate burden time while maintaining sensitivity would benefit clinical experimental studies. We compared a molecular viability (MVA), which measures normalized hsp18 esxA transcript expression, with g...

AIM To validate the analytical performance of a 12-gene molecular assay that predicts distant recurrence for early-stage ER+/HER2- invasive breast cancer as run within a central reference laboratory. MATERIALS & METHODS Formalin-fixed paraffin-embedded breast resections were evaluated by quantitative reverse transcription polymerase chain reaction for the expression of eight target genes, thr...

BACKGROUND This study was designed to compare the detection rates of conventional tumor markers with two molecular diagnostic approaches on blood samples from patients with esophageal squamous cell cancer. MATERIALS AND METHODS Preoperative blood samples were obtained from 44 esophageal cancer patients and were subjected to CEA-specific reverse transcriptase-polymerase chain reaction (RT-PCR)...

Hormone assays are being developed in the laboratory to detect specific molecular markers in nonclinical populations. Epidemiology is increasingly using these assays to improve the precision with which disease processes and exposures can be defined. This growing body of molecular epidemiology requires a high degree of cooperation between the assay developer and the assay user. We draw on our ex...

This report describes the development of a 7-day infectivity assay for bovine leukemia virus (BLV) which is based on the induction of the major internal virion antigen (a protein with a molecular weight of 25,000) in susceptible indicator monolayer cell cultures. In this assay, the antigen is detected in the indicator cells by the immunoperoxidase antibody technique using a specific rabbit anti...

There is widespread interest in the immunostimulatory effects of food-derived substances that are expected to contribute improving human health. Broccoli ( Brassica oleracea var. italica ) has been shown contain potent polysaccharides for developing food materials with immunomodulatory function. In this study, activity previously uncharacterized broccoli was assessed using a silkworm Bombyx mor...

Diabetes mellitus is a metabolic disorder characterized by high blood glucose level in the body. It occurs due to inadequate amount of insulin secreted body or resistance receptors. In present study, Baicalein flavone glycoside was evaluated for its effect on alpha-amylase and alpha-glucosidase enzymes using in-vitro assays extracting respective from whole wheat barley combination with in-silic...

This report describes the development of a 7-day infectivity assay for bovine leukemia virus (BLV) which is based on the induction of the major internal virion antigen (a protein with a molecular weight of 25,000) in susceptible indicator monolayer cell cultures. In this assay, the antigen is detected in the indicator cells by the immunoperoxidase antibody technique using a specific rabbit anti...

This study aimed to investigate the phenolic content, antioxidant activity, cytotoxicity and in vitro wound healing activity of methanolic aqueous extracts Verbascum stachydifolium Boiss & Heldr. var. stachydifolium. Total flavonoid contents were measured using spectrophotometry-based methods. Quantitative analysis selected phenolics was performed by HPLC. The cytotoxic effects on L929 mous...