Abstract Infiltrating tumor-associated macrophages (TAM) are known to impede immunotherapy against glioblastoma (GBM), however, TAMs heterogeneous, and there no clear markers distinguish immunosuppressive potentially immune-activating populations. Here we identify a subset of CD169+ promoting an anti-tumoral microenvironment in GBM. Using single-cell transcriptome analysis, find that human mous...

The uptake of lipoproteins by macrophages is a critical step in the development of atherosclerotic lesions. Cultured monocyte-derived macrophages take up large amounts of native LDL by receptor-independent fluid-phase pinocytosis, either constitutively or in response to specific activating stimuli, depending on the macrophage phenotype. We therefore sought to determine whether fluid-phase pinoc...

Platelet-derived growth factor (PDGF) is secreted by several cells that participate in the process of atherogenesis, including arterial wall monocyte-derived macrophages. Macrophages in human and non-human primate lesions have recently been demonstrated to contain PDGF-B chain protein in situ. In developing lesions of atherosclerosis, macrophages take up and metabolize modified lipoproteins, le...

Infection with a virulent strain of Mycobacterium avium, but not with virulent Mycobacterium tuberculosis or avirulent Mycobacterium smegmatis, induced the formation of nitric oxide by human monocyte-derived macrophages. This process was not affected by lipopolysaccharide or cytokines such as interferon-gamma or tumor necrosis factor alpha. M. avium-induced nitric oxide production was significa...

Granulomas are aggregates of immune cells that can form in various organs. In cutaneous sarcoidosis macrophages and T present inside granuloma cores not able to eliminate the yet unknown antigen arrest an state causing tissue destruction. We aim study switch a protective cell phenotype pathological, disease-driving population within granulomas. Therefore, we use combination direct visualization...

Objective: We previously reported smooth muscle cells (SMCs) represent ?50% of foam in human coronary and ?70% apoE (apolipoprotein E)-deficient mouse aortic atheromas exhibit reduced expression the cholesterol exporter ABCA1 (ATP-binding cassette transporter A1). A major stimulus for is flux out lysosomes, generated by hydrolysis lipoprotein cholesteryl esters LAL (lysosomal acid lipase). In t...

Monocytes and macrophages belong to the mononuclear phagocyte system (MPS). The concept of the MPS is based on the idea that circulating monocytes represent the immediate precursors of tissue macrophages. Recent genetic fate mapping of myeloid cells caused a conceptual frameshift, as it was found that many macrophages are derived from embryonic precursors that seed the tissues before birth and ...

Dermatomyositis (DM) is a chronic, systemic autoimmune disease affecting the skin, muscle, and lungs. Lenabasum, non-psychoactive cannabinoid type 2 receptor (CB2R) agonist, currently being investigated as non-immunosuppressive treatment option for DM. The activation of CB2R has been shown to reduce several key pro-inflammatory cytokines implicated in Our lab previously demonstrated via immunoh...

Acquired resistance in tuberculosis (1), brucellosis (2), listeriosis (3), and salmonellosis (4) is primarily cell mediated. The macrophages, which act as effectors of this type of antimicrobial immunity are derived mainly from circulating monocytes (5). The abundance of macrophages in tissue lesions may be due in part to the state of delayed-type hypersensitivity (DTH) 1 which develops during ...

In immune cell models, macrophages are one of the most frequently used types. THP-1 cells often as model to study macrophage function, however they may act differently from primary human monocyte derived (MDMs). this study, we investigated intrinsic baseline differences between line and MDMs. Additionally, studied difference in response treatment with long-chain polyunsaturated fatty acids (LCP...