Interleukin-1beta (IL-1beta) has been implicated in the pathogenesis of inflammatory diseases of the airway. In this study, we investigated the regulation of MUC2 and MUC5AC expression and of their regulatory mechanisms through cyclooxygenase-2 (COX-2) and prostaglandin E(2) (PGE(2)). Cells activated by IL-1beta showed increased COX-2, MUC2, and MUC5AC expressions at both the mRNA and protein l...

SPOC1 cells, which are a mucin-secreting model of rat airway goblet cells, possess a luminal P2Y2 purinoceptor through which UTP, ATP, and ATPgammaS stimulate secretion with EC50 values of approximately 3 microM. PMA elicits mucin secretion with high EC50 (75 nM) and saturation (300 nM) values. For the first time in airway mucin-secreting cells, the PKC isoforms expressed and activated by a sec...

Chronic neutrophil-predominant inflammation and hypersecretion of mucus are common pathophysiological features of cystic fibrosis, chronic bronchitis, and viral- or pollution-triggered asthma. Neutrophils release elastase, a serine protease, that causes increased mucin production and secretion. The molecular mechanisms of elastase-induced mucin production are unknown. We hypothesized that as pa...

Mucins are glycoproteins synthesized by epithelial cells and thought to promote tumor-cell invasion. Eight human mucin genes have been well characterized: MUC2, MUC5AC, MUC5B, MUC6 map to 11p15.5 and encode secretory gel forming mucins while MUC1, MUC3, MUC4, MUC7 are scattered on different chromosomes and encode membrane-bound or secreted mucins. The expression pattern of the mucin genes is co...

background: patients with primary sjögren’s syndrome (pss) produce functional igg against cholinoreceptor of exocrine glands modifying their activity. the aim of the present work was to demonstrate pss igg antibodies (pss igg) interacting with m 3 muscarinic acetylcholine receptors (machr) of rats submandibular glands that alter mucin release and production via phospholipase c (plc) and cycloox...

The molecular mechanisms of animal cell osmoregulation are poorly understood. Genetic studies of osmoregulation in yeast have identified mucin-like proteins as critical regulators of osmosensitive signaling and gene expression. Whether mucins play similar roles in higher organisms is not known. Here, we show that mutations in the Caenorhabditis elegans mucin-like gene osm-8 specifically disrupt...

The sialosyl-Tn (Sin) antigen is a mucin-associated carbohydrate antigen expressed by a variety of adenocarcinomas. In the colon, ex pression of this antigen has been associated with a poor prognosis, independent of tumor stage or histology. The present study was per formed to determine whether this adverse clinical outcome might be due to an interaction between STn-positive murili and natural ...

PURPOSE To determine whether human conjunctival epithelium expresses any of the human mucin genes designated MUC2 through MUC7. METHOD Northern blot analysis was performed using total RNA isolated from surgically removed conjunctival tissues. Complementary DNA or oligonucleotides to the tandem repeat region of each mucin gene were labeled and hybridized to conjunctival RNA. In situ hybridizat...

Human saliva contains high and low molecular weight mucin glycoproteins, that are distinct. Recently the gene encoding low molecular weight salivary mucin was cloned and designated MUC7, whereas the primary structure of high molecular weight salivary mucin is unclear. Furthermore, the expression patterns of high and low molecular weight salivary mucins in salivary glands have been debated. We h...

PURPOSE Transforming growth factor-β-induced protein (TGFBIp) is highly expressed in the cornea, and mutant TGFBIp induces corneal diseases. However, the function of TGFBIp in cornea epithelium is not fully investigated. Here, we tested the importance of TGFBIp in regulation of gene expression and corneal epithelial cell (CEC) activity. MATERIALS AND METHODS The effect of TGFBIp on CEC activi...