نتایج جستجو برای: multiplex reverse transcriptase pcr rt

تعداد نتایج: 295068  

Journal: :Avian diseases 2004
Holly S Sellers Matthew D Koci Erich Linnemann Laura A Kelley Stacey Schultz-Cherry

A multiplex reverse transcription-polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of two enteric viruses of poultry: turkey enteric coronavirus (TCV) and turkey astrovirus (TAstV). PCR primers were designed to conserved regions within the nucleocapsid gene of TCV and to the polymerase gene of TAstV-2. The primer pairs were successfully used in a multiplex R...

2018
Dongyang Cai Ole Behrmann Frank Hufert Gregory Dame Gerald Urban

The full potential of the real-time reverse transcription polymerase chain reaction (RT-PCR) as a rapid and accurate diagnostic method is limited by DNA polymerase inhibitors as well as reverse transcriptase inhibitors which are ubiquitous in clinical samples. rTth polymerase has proven to be more resistant to DNA polymerase inhibitors present in clinical samples for DNA detection and also exhi...

Journal: :Cancer research 1993
T Deguchi T Doi H Ehara S Ito Y Takahashi Y Nishino S Fujihiro T Kawamura H Komeda M Horie

We have developed a highly sensitive method for detecting prostate cancer cells using reverse transcriptase-polymerase chain reaction (RT-PCR) with primers specific for prostate-specific antigen gene. Forty-four lymph nodes obtained from 22 patients with prostate cancers were analyzed by RT-PCR to detect metastatic prostate cancer cells. RT-PCR could detect prostate-specific antigen mRNA in fiv...

2017
Yong Yan Jian-Yong Luo Yin Chen Heng-Hui Wang Guo-Ying Zhu Pei-Yan He Jin-Lei Guo Yong-Liang Lei Zhong-Wen Chen

We utilized one-step multiplex reverse transcription-PCR (RT-PCR) and Luminex xMAP technology to develop a respiratory multiplex liquid-chip assay (rMLA) for simultaneous detection of 6 common respiratory viruses, including influenza virus type A (FluA) and type B (FluB), para-influenza virus type 3 (PIV-3), respiratory syncytial virus (RSV), human metapneumovirus (MPV) and a threatening virus ...

Journal: :iranian journal of microbiology 0
f abbasian virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. h tabatabaie virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. m sarijloo virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. s shahmahmoodi virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. a yousefi virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran. t saberbaghi virology division, pathobiology department, school of public health, tehran university of medical sciences, tehran, iran.

background and objectives : each year, enteroviruses infect millions of people and cause different diseases. the agents are usually detected using cell culture. rd (rhabdomyosarcoma) and l20b (l cells) are among the recommended cells by the world health organisation (who) for this purpose. even though cell culture is the most common method used in diagnosing enteroviruses in stool specimens, th...

Journal: :Memorias do Instituto Oswaldo Cruz 2008
Leandro Rua Ribeiro Rafael Souto de Oliveira Giuberti Débora Maria Pires Gonçalves Barreira Ketene Wernek Saick José Paulo Gagliardi Leite Marize Pereira Miagostovich Liliana Cruz Spano

Viruses are the leading cause for hospitalization due to gastroenteritis worldwide. Group A rotaviruses (RV) are the most prevalent and are assorted in glycoproteins (G) and protease sensitive (P) dual genotypes based on polymorphic genes that encode the external VP7 and VP4 capsid proteins, respectively. Noroviruses (NoV) have increasingly answered by sporadic gastroenteritis. This study aimed...

Journal: :Methods in molecular biology 2010
Cristina Hartshorn Lawrence J Wangh

LATE-PCR is an optimized form of asymmetric PCR that efficiently generates high levels of single-stranded DNA amplicons. Single-stranded amplicons are advantageous because, as shown in this chapter, they can be probed at low temperature(s) with one or more probes. Based on its properties, LATE-PCR is also useful for constructing multiplex assays. Viral RNA or RNA present in cells can be detecte...

Journal: :Journal of clinical microbiology 2005
Pia Jokela Päivi Joki-Korpela Marita Maaronen Virpi Glumoff Timo Hyypiä

A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all human picornaviruses were covered. The amplicons, which represented the 5' unt...

Journal: :Southern African Journal of Infectious Diseases 2021

Background: Serology testing is an important ancillary diagnostic to the reverse transcriptase polymerase chain reaction (RT-PCR) test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We aimed evaluate performance of Roche Elecsys™ chemiluminescent immunoassay (Rotkreuz, Switzerland), that detects antibodies against SARS-CoV-2 nucleocapsid antigen, at academic laboratory in Sou...

Journal: :Revista De La Facultad De Agronomia De La Universidad Del Zulia 2022

The glyoxalase system plays an important role in various physiological processes plants when they are subjected to different types of stress, whether physical, chemical or biological. Aspergillus flavus is aflatoxin-producing fungus that contaminates dry grains, leading a gradual deterioration the grains and significant reduction their nutritional value. objective present study was evaluate act...

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