The population of many splendid orchids is reducing from their natural habitats at an alarming rate and their conservation is becoming a matter of global concern. Asymbiotic seed germination has been applied for ex situ conservation of rare, endangered and threatened orchid taxa and could provide rapid means their multiplication. In the present study reported here, seeds of an epiphytic and rar...

An effective and improved plant regeneration system was successfully developed using shoot tip explants taken from a two years old mature plant of Cassia angustifolia. The effect of different cytokinins, [6-benzyladenine (BA), Kinetin (Kin) and thidiazuron (TDZ)] at different concentrations (0.5-10 μM) were evaluated as augmented with Murashige and Skoog (MS 1962) medium. Among all the cytokini...

Stevia rebaudiana Bertoni is a Paraguayan perennial antidiabetic herb belongs to Asteraceae family. The Leaves of stevia are about 300 times sweeter than sugar with no calories, that are the source of diterpene glycosides such as stevioside and rebaudioside A. Tissue culture is a rapid tool for propagation of stevia. In this research we studied the effect of different concentrations of mannitol...

Shoot organogenesis and plant establishment has been achieved for Balanites aegyptiaca (L.) Del. from excised root explants. Young root explants obtained from 4 weeks old aseptic seedlings were used for the induction of direct shoot regeneration by incorporating various cytokinins either singly or in combination with auxins in Murashige and Skoog (MS) basal medium. Root explants cultured on MS ...

An efficient protocol is suggested for in vitro culture of five Phalaenopsis hybrids obtained by hand-cross-pollination of three commercial hybrids Calgary, Ankara, and Kendall. Four nutrient media- namely half-strength Murashige and Skoog, Knudson, Phytamax containing activated charcoal, and Mitra– once supplemented (with coconut water, peptone, or both), and once without any supplement were c...

Shoot tips from actively growing, greenhouse maintained plants of Kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on Murashige and Skoog (MS) basal medium supplemented with NAA and BA, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. Results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost ...