In this study 253 serum samples from cattle showing respiratory disorders were tested by confirmatory Western Immunoblotting test (IBT), complement fixation (CFT) and competitive enzyme linked immunosorbent assay (c-ELISA). Two (0.8%) out of found to be positive two doubtful CFT while 7 (2.8%) as 25 (9.9%) them c-ELISA. On the other hand, a core profile antigenic bands needed identified at 110,...

Intraclonal antigenic variation in pathogenic mycoplasma species is considered an important feature of host-pathogen interaction. Such intraclonal protein variation was observed for the interaction of Mycoplasma mycoides subsp. mycoides Small Colony, the agent of contagious bovine pleuropneumonia, with mAb 3F3. Colony immunostaining allows the definition of 3F3 ON- and 3F3 OFF-type variants, wh...

In this study, an immunoproteomic approach was used to identify immunodominant proteins from Mycoplasma mycoides subsp. capri isolates. Membrane proteins, extracted through TX-114 phase partitioning, were separated using mono- and two-dimensional electrophoresis and detected by Western blotting with pooled sera from naturally infected goats. A total of 27 immunoreactive spots, corresponding to ...

www.sciencemag.org SCIENCE VOL 329 2 JULY 2010 5 COVER Electron micrograph of Mycoplasma mycoides JCVI-syn1.0 cells (magnifi cation ~25,000×). These cells were produced following transplantation of a 1.08–megabase pair synthetic M. mycoides genome into M. capricolum recipient cells. The cells are controlled by the synthetic genome, exhibit the expected phenotypic properties, and are capable of ...

  Mycoplasma capricolum subsp capricolum (Mcc) is one of the etiological agents of contagious agalactia(C.A) in goats which can cause significant economic losses. The aim of this study was to detect Mcc from goats of Qom province in Iran. A total of 111 samples were collected from suspected goats to C.A and cultured in PPLO broth supplemented for Mcc isolation. The bacteria DNAs were extracted ...

The measurement of lactate dehydrogenase activity has been used to evaluate the growth of the T(1) vaccine strain of Mycoplasma mycoides var. mycoides in broth during the production of vaccine. Estimations were made during incubation at 37 C and storage at 4 C. The results were compared with titers of the vaccine obtained by two conventional methods of assessing viability titers: the counting o...

mycoplasma is an important respiratory tract pathogen worldwide, causing respiratory tract infection in small ruminants ( sheep and goats). it is a bacterium that causes acute respiratory illness ranging in severity from mild respiratory illness to severe pneumonia. mycoplasmosis in small ruminants is a serious and major problem in iran. this study was determined to isolate mycoplasma species a...