The phytotoxin coronatine (COR) promotes various aspects of Pseudomonas syringae virulence, including invasion through stomata, growth in the apoplast, and induction of disease symptoms. COR is a structural mimic of active jasmonic acid (JA) conjugates. Known activities of COR are mediated through its binding to the F-box–containing JA coreceptor CORONATINE INSENSITIVE1. By analyzing the intera...

Dietary R-sulforaphane is a highly potent inducer of the Keap1/Nrf2/ARE pathway. Furthermore, sulforaphane is currently being used in clinical trials to assess its effects against different tumour processes. This study reports an efficient preparation of enantiopure R-sulforaphane based on the enzymatic hydrolysis of its natural precursor glucoraphanin. As an alternative to broccoli seeds, we h...

Isothiocyanates (ITCs) are naturally occurring molecules belonging to highly reactive organosulphur synthons, with the general structure R–N=C=S. The precursor molecule glucosinolate anions are hydrolyzed enzymatically (under the effect of myrosinase enzymes) or unenzymatically to produce nitriles or isothiocyanates depending upon conditions such as pH and temperature. Brassicaceae  Family is k...

Assessment of the oral use of indole-3-carbinol (I3C) as a chemoprotective compound has not sufficiently considered the chemical instability of I3C. This review addresses the question of whether I3C is directly active in its own right or only serves as a precursor, with all of the biological responses coming from reaction products arising in culture media and in the presence of stomach acid. Be...

Corresponding author: Hilmer Sørensen, Department of Natural Sciences, Biochemistry and Natural Product Chemistry, The Royal Veterinary and Agricultural University. Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. Tel. +45 35282432; fax: +45 35282398; e-mail [email protected]; phone INTRODUCTION Glucosinolates are alkyl-N-hydroximine sulphate esters with a β-Dthioglucopyranoside group attached t...

The myrosinase enzyme hydrolyzes glucosinolates, among which is glucoraphanin, the precursor of anticancer isothiocyanate sulforaphane (SFN). main source glucoraphanin Brassicaceae; however, its natural concentration relatively low, limiting availability SFN. An option to obtain SFN exogenous production, through enzymatic processes and under controlled conditions, allowing complete conversion W...