Abstract BACKGROUND Pathway-specific targeted agents have failed to improve outcome in patients with high grade gliomas. Tumor recurrence is driven by glioma heterogeneity and emergence of therapy-resistant subpopulations. We previously reported that metabolic targeting NAMPT, the rate limiting enzyme NAD salvage synthesis pathway, circumvents induces cytotoxicity mitochondrial membrane depolar...

NAD + has previously been shown to regulate TNF-α synthesis and TNF-α has been shown to regulate NAD + homeostasis, thus providing a link between a pro-inflammatory response and redox status. Despite the well-established link between TNF-α and NAD + , the mechanism as to how NAD + modulates TNF-α release is not fully understood. To achieve this, this link was investigated using THP-1 cell line-...

Background: Objectives 1- To analyze the levels of NAD + and genes involved in its metabolism Rheumatoid Arthritis (RA) patients. 2- evaluate effect anti-TNF therapy metabolism. 3- explore mechanistically anti-inflammatory effects boosters leukocytes from RA Methods Plasma PBMCs were purified 100 patients 50 healthy donors (HDs). NAD+ determined by using NAD/NADH-Glo Assay. Gene expression anal...

Beyond its well-described role in cellular metabolism, intracellular nicotinamide adenine dinucleotide (NAD) levels have been shown to affect the enzymatic activity of a series of NAD-dependent enzymes, influencing biological responses such as cell survival and inflammation. Nicotinamide phosphoribosyl transferase activity has been shown to be essential for maintaining adequate intracellular NA...

Purpose: We sought to identify predictive biomarkers for a novel nicotinamide phosphoribosyltransferase (NAMPT) inhibitor. Experimental Design:We use a NAMPT inhibitor, GNE-617, to evaluate nicotinic acid rescue status in a panel ofmore than 400 cancer cell lines.Using correlative analysis andRNA interference (RNAi), we identify a specific biomarker for nicotinic acid rescue status. We next det...

Pre-B cell colony-enhancing factor (PBEF) is a highly conserved pleiotropic protein reported to be an alternate ligand for the insulin receptor (IR). We sought to clarify the relationship between PBEF and insulin signaling by evaluating the effects of PBEF on the localization of the IRβ chain to lipid rafts in A549 epithelial cells. We isolated lipid rafts from A549 cells and detected the IR by...