An alternative process for the analysis of mixture library components for their potential as selectors for chiral chromatography is described. The procedure involves the immobilization of each enantiomer of the target racemic analyte to silica gel, followed by incubation of each resulting stationary phase with a mixture library. The adsorbed library components on the two stationary phases are t...

In forensics, the analysis of ink writings from documentation is required for authentication or crime analysis. Ten organic ink dye components typically found in ink pens were separated using the Agilent 1290 Infinity LC System and quantified using the Agilent 6140 single quadrupole LC/MS System. The dyes were separated in less than 3.5 min using a sub-2-μm, 30 mm column. Analysis of ink markin...

We present a liquid chromatography-mass spectrometry (LC-MS)-based method for comprehensive quantitative identification of post-transcriptional modifications (PTMs) of RNA. We incorporated an in vitro-transcribed, heavy isotope-labeled reference RNA into a sample RNA solution, digested the mixture with a number of RNases and detected the post-transcriptionally modified oligonucleotides quantita...

The applicability of Supercritical Fluid Chromatography (SFC) can be substantially enhanced by coupling SFC to Mass Spectrometric detection (MSD). In this Technical Overview, two instrument configurations are described that can be used to connect the Agilent 1260 Infinity Analytical SFC to a mass spectrometer either using an atmospheric pressure chemical ionization (APCI) or an electrospray (ES...

Modern software platforms enable the analysis of shotgun proteomics data in an automated fashion resulting in high quality identification and quantification results. Additional understanding of the underlying data can be gained with the help of advanced visualization tools that allow for easy navigation through large LC-MS/MS datasets potentially consisting of terabytes of raw data. The updated...

Two unknown impurities were detected in the cefazolin sodium bulk drug substance using gradient reversed-phase high-performance liquid chromategraphy (HPLC). These impurities were isolated by preparative HPLC and characterized by using spectroscopic techniques like LC-MS, LC-MS/MS, 1D, 2D NMR, and FT-IR. Based on the spectral data, the impurities have been characterized as N-(2,2-dihydroxyethyl...

A new degradant of Nafcillin Sodium was found at a level of 1.8% w/w during the gradient reversed-phase HPLC analysis in stability storage samples. This impurity was identified by LC-MS and was characterized by (1)H-NMR, (13)C-NMR, LC/MS/MS, elemental analysis, and IR techniques. Based on the structural elucidation data, this impurity was named as N-[(2S)-2-carboxy-2-{[(2-ethoxynaphthalen-1-yl)...

A simple, specific, precise and accurate reversed phase liquid chromatographic (RP-LC) method has been developed for the determination of ascorbic acid in different food additives. The chromatographic separation was achieved on a LiChrosorb C18, 250 mm x 4.6 mm, 5 μm column at a detector wavelength of 230 nm and a flow rate of 1.5 ml/min. The mobile phase was composed of acetonitrile and water ...

A new, highly selective and sensitive scan function for quantification of 11-nor-9-carboxy-D-tetrahydrocanabinol (THC carboxylic acid) in hair is presented. Hair is a very complex matrix and high background and/or interferences around the expected retention time for THC carboxylic acid are observed in the main MRM traces. The ability to use fragments of second order, MS (MS/MS/MS), will show a ...

A rapid method was developed for the evaluation of forskolin in Coleus forskohlii Briq. (Lamiaceae). Forskolin was quantitated in the root and stem of dried C. forskohlii and in 17 market products by reversed-phase liquid chromatography (LC) with a photodiode array detector at 210 nm. The temperature was held constant at 30 degrees C, and the retention time of forskolin was approximately 6.8 mi...