The period protein (PER) is an essential component of the circadian clock in Drosophila melanogaster. Although PER-containing pacemaker cells have been previously identified in the brain, the neuronal network that comprises the circadian clock remained unknown. Here it is shown that some PER neurons are also immunostained with an antiserum against the crustacean pigment-dispersing hormone (PDH)...

For the lymphocytic descendants of the hematopoietic differentiation pathway, characteristic gene rearrangements result in deletions of signif icant portions of chromosome regions specifying lymphocyte-specific gene products on either or both chromosomes of each involved pair. Molecular mechanisms facilitating the rearrangement/deletion events have been elegantly and fruitfully elaborated in th...

In this study, immunotoxins containing monoclonal anti-human T-cell leukemia antibodies are shown to be capable of specific killing of human T-leukemia cells in vitro. These immunotoxins were prepared by conjugating ricin A chain (RIA) with our recently generated murine monoclonal anti bodies, SN1 and SN2, the latter of which was obtained from a hybridoma clone N6/D11 described previously by Ne...

Phosducin is a cytosolic protein predominantly expressed in the retina and the pineal gland that can interact with the fty subunits of guanine nucleotide binding proteins (G proteins) and thereby may regulate transmembrane signaling. A cDNA encoding a phosducin-like protein (PhLP) has recently been isolated from rat brain [Miles, M. F., Barhite, S., Sganga, M. & Elliott, M. (1993) Proc. Nati. A...

The cytochemical technique using the in situ precipitation of orthophosphate ions liberated specifically by the aspartate carbamoyltransferase (ATCase) (EC 2.1.3.2) reaction indicated that in Saccharomyces cerevisiae this enzyme is confined to the nucleus. This observation is in accordance with the result reported by Bernhardt and Davis (1972), Proc. Natl. Acad. Sci. U. S. A. 69:1868-1872) on N...

Tropospheric ozone, lot-med from nonlinear reactions between VOC5 and nitrogen oxides (NOr), is a primarr constituent of urban smog (1). Estimates of VOC control costs needed to attain the National Ambient Air Quality Standard (NAAQS) for ozone of 0.12 parts per million are on the order of billions of dollars per year, and in the most severely impacted region~, the necessary control technologie...

A basic protein from the dimorphic fungus Mucor racemosus, found to be highly methylated, is shown to be protein synthesis elongation factor la. This protein is the most abundant protein in hyphal cells but is less abundant in yeast cells. It is posttranslationally methylated with the formation of mono-, di-, and trimethyllysine at as many as 16 sites. Nearly 20% of the 44 lysine residues of el...

Crystals of the bovine milk protein lactollin yield x-ray diffraction data extending to a resolution of 2.8 A. Lactollin is a bovine analogue of #2-microglobulin, a protein that is homologous in amino acid sequence to the constant domains of immunoglobulins and is the light chain of the human and murine major histocompatability antigens. The protein crystallizes in the orthorhombic space group ...

References and Notes 1. S. Fields, Y. Kohara, D. J. Lockhart, Proc. NatI. Acad. Sci. U.S.A. 96, 8825 (1999); A. Goffeau et al., Science 274, 546 (1996). 2. P. Ross-Macdonald et al., Nature 402, 413 (1999); J. L. DeRisi, V. R. lyer, P. 0. Brown, Science 278, 680 (1997); E. A. Winzeler et at., Science 285, 901 (1999); P. Uetz et al., Nature 403, 623 (2000); T. Ito et al., Proc. Natl. Acad. Sci. U...

Automated solid waste processing is a very compli­ cated task: complicated equipment should be consid­ ered despite the fact that it can cause inordinate downtime if misapplied. Examples from laboratory re­ search and development are compared with reports of �eld experience to produce guidelines for distinguish­ mg between the types of equipment complexity that are and are not acceptable. A ren...