Examination of antinuclear antibody (ANA) is used in diagnosis systemic autoimmune diseases, and the indirect immunofluorescence (IIF) assay using HEp-2 cells gold standard method. allows detection multiple target antigen-directed autoantibodies. The guide “The International Consensus on ANA Patterns (ICAP)”, characterizes patterns into three groups: nuclear, cytoplasmic, mitotic. majority thes...

Objective: To run a comparison between specificity, sensitivity, PPV (positive predictive value) (PPV) & NPV (negative of the three varied H. pylori detection methods used in study. Materials and Methods: It is retrospective descriptive study that was carried out at Histopathology wing Pathology Department, Sahiwal Medical College, Sahiwal. comprised entire cases gastritis which were histop...

Background and Objective: Diagnosis of Hirschsprung’s disease (HD) as the most common cause of neonatal intestinal obstruction is based on the presence of aganglionosis from seromuscular or full thickness biopsy. Due to the complication of full thickness or seromuscular rectal biopsy, mucosal-sub mucosal...

A punch biopsy of the axillary region was performed for histopathological study, staining with hematoxylin-eosin (Figure 2), periodic acid-Schiff (PAS), and Congo red. The histological findings included papillomatosis, orthokeratotic hyperkeratosis and epidermal acanthosis, focal atrophy of the granular layer, and mild hyperpigmentation of the basal layer of the epidermis. There was a moderate ...

Comparisons were made in Lusaka and in London between manual staining and staining in an automatic machine with auramine-phenol of direct smears of sputum and other types of specimen for acid-fast bacilli. No evidence was obtained of carry-over of acid-fast bacilli from positive to negative smears during machine staining. There was improved contrast between bacilli and the background in smears ...